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A new type of highly selective aptamer-labeled fluorescent silica nanoparticles[Apt-tris(2,20-bipyridyl)ruthenium(II)@SiO2NPs]were prepared through the reverse microemulsion method by using prolonged fluorescence lifetime ruthenium complexes of tris(2,20-bipyridyl)ruthenium(II)(Ru(bpy)32?)as the source of the fluorescence for cellular prion protein imaging.Investigations showed that the newly prepared Ru(bpy)32?@SiO2NPs possessed superior advantages of strong fluorescence,low toxicity,and easy surface modification for bioconjugation.Cell imaging experiments indicated that AptRu(bpy)32?@SiO2NPs had great tendency to human bone marrow neuroblastoma cells(SK-N-SH cells),since they can express large amount of prion protein on the surface of the cell,while in HeLa cells this phenomenon disappeared for the reason that HeLa cells cannot express prion proteins.
A new type of highly selective aptamer-labeled fluorescent silica nanoparticles [Apt-tris (2,20-bipyridyl) ruthenium (II) @SiO2NPs] were prepared through the reverse microemulsion method by prolonged fluorescence lifetime ruthenium complexes of tris -bipyridyl) ruthenium (II) (Ru (bpy) 32®) as the source of the fluorescence for cellular prion protein imaging. Investigations showed that the newly prepared Ru (bpy) 32® @ SiO2 NPs possessed superior superior of strong fluorescence, low toxicity , and easy surface modification for bioconjugation. Cell imaging experiments that that AptRu (bpy) 32 ~ @ SiO2NPs had great tendency to human bone marrow neuroblastoma cells (SK-N-SH cells), since they can express large amount of prion protein on the surface of the cell, while in HeLa cells this phenomenon disappeared for the reason that HeLa cells can not express prion proteins.