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目的 :观察肝疒徵 口服液含药血清对转化生长因子 (TGFα)诱导人肝癌细胞增殖和凋亡的影响。方法 :采用血清药理学方法 ,以病理鼠血清作对照 ,应用MTT比色法观察中药鼠血清对TGFα诱导人肝癌细胞SMMC 772 1增殖的抑制作用。用流式细胞术检测中药血清对肝癌细胞凋亡和细胞周期的影响。结果 :中药血清对TGFα诱导的肝癌细胞SMMC 772 1增殖有明显的抑制作用 ,抑制效应呈时间依赖性。中药血清能促进肝癌细胞凋亡 ,使细胞滞留于G0 1 期 ,降低S期细胞百分比和增殖指数。结论 :肝疒徵 口服液含药血清能够抑制TGFα诱导的人肝癌细胞增殖 ,促进其凋亡。
Objective : To observe the effect of serum containing liver and phlegm granules on the proliferation and apoptosis of human hepatocellular carcinoma cells induced by transforming growth factor (TGFα). Methods : Using serum pharmacology, using pathological mouse serum as a control, MTT colorimetric method was used to observe the inhibitory effect of traditional Chinese medicine mouse serum on the proliferation of SMMC 772 1 induced by TGFα. Flow cytometry was used to detect the effect of serum of Chinese medicine on apoptosis and cell cycle of hepatoma cells. RESULTS: The serum of traditional Chinese medicine had obvious inhibitory effect on the proliferation of SMMC 772 1 cells induced by TGFα, and the inhibitory effect was time-dependent. Serum of traditional Chinese medicine can promote the apoptosis of hepatocellular carcinoma cells, so that the cells remain in the G0 phase, and the percentage of cells in the S phase and the proliferation index are decreased. Conclusion : The serum containing liver phlegm can inhibit the proliferation of human hepatoma cells induced by TGFα and promote its apoptosis.