论文部分内容阅读
目的：观察、探讨硫酸乙酰肝素蛋白聚糖在正常及有动脉粥样硬化病变的人冠状动脉内的分布和对巨噬细胞脂质摄入的影响及其与动脉粥样硬化形成的关系。方法：应用免疫组化染色观察正常和动脉粥样硬化病变的人冠状动脉内硫酸乙酰肝素蛋白聚糖的分布；应用酶－荧光法，检测培养的巨噬细胞内胆固醇含量。结果：（１）硫酸乙酰肝素蛋白聚糖主要分布于正常冠状动脉内膜近腔面的１／３处，多定位于内皮基底膜及内膜细胞的细胞膜周围；于动脉粥样硬化病变（脂纹及斑块）内其分布密度下降，尤其是在病变深层的泡沫细胞周围分布稀少。（２）硫酸乙酰肝素蛋白聚糖能抑制巨噬细胞内脂质的聚集。结论：动脉内膜中硫酸乙酰肝素蛋白聚糖分布的减少可能与巨噬细胞易于摄入脂质转变为泡沫细胞有关，对动脉粥样硬化早期病变的形成和发展可能具有重要作用。
OBJECTIVE: To investigate the distribution of heparan sulfate proteoglycan in human coronary arteries with normal and atherosclerotic lesions and its effect on the lipid uptake of macrophages and its relationship with the formation of atherosclerosis. Methods: Immunohistochemical staining was used to observe the distribution of heparan sulfate proteoglycan in normal and atherosclerotic lesions in human coronary arteries. The cholesterol content in cultured macrophages was detected by enzyme-fluorescence method. Results: (1) Heparan sulfate proteoglycan mainly located in 1/3 of the normal coronary intima near the surface of the intima, and localized in the membrane of endothelium and endometrial cells. In atherosclerotic lesions Wrinkles and plaques) within the distribution of its density decreased, especially in the lesions around the thin foam cells distributed scarce. (2) Heparan sulfate proteoglycan can inhibit lipid accumulation in macrophages. CONCLUSION: The decrease of heparan sulfate proteoglycan distribution in arterial intima may be related to the easy uptake of lipids into foam cells by macrophages and may play an important role in the formation and development of early atherosclerotic lesions.