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将聚合酶链式反应（ＰＣＲ）技术与光镜、电镜下的形态学观察相结合，对６３例女性生殖道疣样病损活检标本进行综合研究。ＰＣＲ检测结果：在２６例新鲜组织中，人乳头状瘤病毒（ＨＰＶ）６／１１ｂ型阳性者２１例（８０．８％）；在１２例石蜡包埋组织中，ＨＰＶ６／１１ｂ阳性者７例（５８．３％），总阳性率为７３．７％。光镜观察：２１例ＨＰＶ阳性病例中，１９例显示典型的尖锐湿疣病变，另２例未发现典型的挖空细胞，基底细胞增生也不明显；ＨＰＶ阴性病例绝大部分无典型尖锐湿疣表现，但有１例在中、表层也出现了成堆的挖空细胞。电镜观察发现：ＨＰＶ阳性病例的超微结构显示一定程度的规律性。提示：尖锐湿疣系ＨＰＶ感染所引起，将光镜、电镜的形态学观察与ＰＣＲ技术检测ＨＰＶ相结合，对之进行综合诊断，可减少尖锐湿疣的误诊与漏诊；ＨＰＶ阳性的湿疣组织中，病毒颗粒的出现机率极小，研究中发现的与病毒感染有关的超微病变，可试作电镜下诊断尖锐湿疣的依据
Polymerase chain reaction (PCR) technology and light microscopy, electron microscopy combined morphological observation of 63 cases of female genital wart-like lesion biopsy specimens for comprehensive study. The results of PCR showed that among 26 fresh tissues, 21 (80.8%) were positive for HPV type 6 / 11b and 7 were positive for HPV6 / 11b in 12 paraffin-embedded tissues (58.3%), the total positive rate was 73.7%. Light microscopy: 21 cases of HPV-positive cases, 19 cases showed a typical condyloma acuminata lesions, the other two cases found no typical hollow cells, basal cell hyperplasia is not obvious; HPV-negative cases, the vast majority of typical no genital warts, However, in one case, piles of hollow cells appeared on the surface. Electron microscopy revealed that the ultrastructure of HPV-positive cases showed a certain degree of regularity. Tip: Condyloma acuminatum caused by HPV infection, the light microscope, electron microscopy and HPV detection of HPV morphology combined observation of its comprehensive diagnosis can reduce the misdiagnosis and misdiagnosis of genital warts; HPV-positive genital warts, the virus The probability of occurrence of particles is extremely small, the study found that the virus infection-related ultrafine lesions, can be used for diagnosis of genital warts on the basis of electron microscopy