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目的 了解肝癌患者乙型肝炎病毒 (HBV)DNA含量及其与C基因启动子 (BCP)基因变异的关系。方法 采用PCR荧光实时定量技术检测 114例肝癌患者及 10 0例非肝癌乙肝患者HBVDNA含量。采用PCR -微板核酸分子杂交ELISA技术对肝癌及乙肝患者进行BCP基因变异检测。结果 肝癌患者 4 8%HBVDNA阳性 ,平均拷贝量为 4 .7× 10 6拷贝 /ml。BCP区域 176 2、176 4位突变率为 2 7% ,且BCP变异的患者HBV拷贝量明显大于非变异患者。 10 0例非肝癌乙肝患者HBVDNA阳性率 4 1% ,平均拷贝量 3.8× 10 5拷贝 /ml,BCP基因突变率为 8% ,肝癌患者的BCP基因突变率明显高于乙肝患者。结论 HBV感染可能是导致肝癌发生的重要原因 ,HBVBCP变异可能与病变程度有关。
Objective To investigate the DNA content of hepatitis B virus (HBV) in patients with hepatocellular carcinoma and its relationship with the gene mutation of C gene promoter (BCP). Methods The real-time quantitative PCR detection of HBVDNA in 114 patients with hepatocellular carcinoma and 100 patients with non-hepatocellular carcinoma was performed. BCP gene mutation was detected in patients with hepatocellular carcinoma and hepatitis B by ELISA - microplate nucleic acid hybridization ELISA. Results Forty-eight percent of patients with HCC were positive for HBV DNA with an average copy size of 4.7 × 10 6 copies / ml. In the BCP region 176 2,176 4-bit mutation rate of 27%, and BCP mutation in patients with HBV copies was significantly greater than non-variant patients. The HBVDNA positive rate was 41% and the average copy number was 3.8 × 10 5 copies / ml in 10 0 non-HCC patients. The mutation rate of BCP gene was 8%. The mutation rate of BCP gene in HCC patients was significantly higher than that of hepatitis B patients. Conclusion HBV infection may be an important cause of HCC. The variation of HBV BCP may be related to the severity of HCC.