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目的 :观察外源性溶血磷脂酰胆碱 (L PC)对离体大鼠工作心脏的损伤作用及缺血预处理 (IP)对离体大鼠心脏的保护作用。方法 :制备离体大鼠工作心脏模型。随机分为 4组 :C组 :用 K- H液连续灌注 35 min; 组 :停灌注 40 m in,再灌注 30 m in;L 组 :用含 5 μmol/L L PC的 K- H液灌注 5 min,再用正常 K- H液灌注 30 min;P组 :停灌注 5 m in后再灌注 5 min,重复 3次 ,然后重复 L 组全过程。结果 :再灌后 ,L 组和 I组心功能均明显下降 ,室颤发生率提高 ,灌注液中乳酸脱氢酶 (L DH)和丙二醛 (MDA)含量明显升高 ,心肌组织中超氧化物歧化酶 (SOD)活力明显降低。与 L 组相比 ,P组心功能指标明显升高 ,L DH和 MDA含量明显降低 ,SOD活力较高 ,无室颤发生。结论 :L PC可导致与经典方法类似效果的类缺血再灌注损伤作用 ,IP对 L PC损伤有保护作用。
OBJECTIVE: To observe the effects of exogenous lysophosphatidylcholine (L PC) on the working heart of isolated rat hearts and the protective effect of ischemic preconditioning (IP) on the isolated rat heart. Methods: Working heart model was made in vitro. Rats in group C were randomly divided into 4 groups: Group C: continuous perfusion with K-H solution for 35 min; group: perfusion stopped 40 m in, reperfusion for 30 m in; Group L: perfusion with K-H solution containing 5 μmol / min, followed by normal K-H fluid perfusion for 30 min; P group: stop perfusion 5 m in and then perfusion 5 min, repeat 3 times, and then repeat the whole process of L group. Results: After reperfusion, the cardiac function of L group and I group were significantly decreased, the incidence of ventricular fibrillation increased, the content of L DH and MDA significantly increased, The activity of dismutase (SOD) was significantly reduced. Compared with L group, P group significantly increased cardiac function, L DH and MDA content was significantly lower, higher SOD activity, no occurrence of ventricular fibrillation. CONCLUSION: L-PC can induce ischemia-reperfusion-like injury similar to classical methods, and IP has a protective effect against L PC injury.