Objective:To investigate the effect of Zi Chong Granules(资冲颗粒)on estradiol(E_2)synthesis in thecultured ovarian granular cells of the mice,so as to probe into the mechanism of the drug in promotingfollicular development.Methods:Testosterone(T)with different concentration were added to thecultured ovarian granular cells,and 24 and 48 hours later the enzyme immunoassay(EIA)was used todetermine the required concentration of T and time for E_2 synthesis;Zi Chong Granules,its activecomponents for tonifying the kidney and removing blood stasis,and the mouse serum containing Zi ChongGranules were respectively added to the medium of the cultured mouse ovarian granular cells and themixtures were cultured for 48 hours to determine the E_2 concentration in various cultures,with insulingrowth factor-1(IGF-1),follicle-stimulating hormone(FSH)and NJin Dan(女金丹丸 Golden Pill forWomen)as positive control drugs.Results:Zi Chong Granules and NJin Dan(were found to be able toincrease the E_2 level in the medium of the cultured ovarian granular cells to a certain extent,and the activecomponents for tonifying the kidney or those for removing the blood stasis did not increase the E_2 level,but the mouse serum containing Zi Chong Granules could significantly increase the level of E_2.Conclusion:To promote the E_2 synthesis in the ovarian granular cells is one of mechanisms of Zi ChongGranules in stimulating follicular development. Objective: To investigate the effect of Zi Chong Granules (资冲粒) on estradiol (E_2) synthesis in the cultured culture granular cells of the mice, so as to probe into the mechanism of the drug in promotingfollicular development. Methods: Testosterone (T) With different concentration were added to thecultured culture granular cells, and 24 and 48 hours later the enzyme immunoassay(EIA) was used todetermine the required concentration of T and time for E_2 synthesis;Zi Chong Granules,its active component for tonifying the kidney and removing blood Stasis,and the mouse serum containing Zi ChongGranules were respectively added to the medium of the cultured mouse cultured granular cells and the mixtures were cultured for 48 hours to determine the E_2 concentration in various cultures, with insulingrowth factor-1(IGF-1),follicle -stimulating hormone (FSH) and NJin Dan (Golden Pill for Women) as positive control drugs.Results: Zi Chong Granules and NJin Dan (were found to be able to increse the E_ 2 level in the medium of the cultured culture granular cells to a certain extent,and the active components for fornifying the kidney or those for removing the blood stasis did not increase the E_2 level,but the mouse serum containing Zi Chong Granules could significantly increase the level Of E_2.Conclusion:To promote the E_2 synthesis in the ovarian granular cells is one of mechanisms of Zi ChongGranules in stimulating follicular development.