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本实验检测了一种恶性疟原虫多价重组候补疫苗在小鼠体内引起的体液免疫和细胞免疫反应。该重组疫苗包含了MSA1、MSA2、RESA上的T细胞和／或B细胞刺激位点及IL－1、TT上的T细胞刺激位点；尤其是含有Spf66的序列。经重组疫苗免疫后，BALB／c小鼠和C57BL／6小鼠均产生了明显的抗重组疫苗的抗体，且经重组疫苗免疫的BALB/c小鼠脾细胞在体外经重组疫苗刺激后发生了明显的增殖，并产生了明显的IL－2。以上结果提示该候补疫苗具有免疫原性，且具有一定的T细胞刺激能力和一定的广谱识别能力，其能否有效地刺激人淋巴细胞还需进一步研究。
This experiment tested a humoral and cellular immune response caused by a Plasmodium falciparum multivalent recombinant vaccine in mice. The recombinant vaccine contains T cell and / or B cell stimulation sites on MSA1, MSA2, RESA and T cell stimulation sites on IL-1 and TT; in particular, sequences containing Spf66. After immunization with the recombinant vaccine, BALB / c mice and C57BL / 6 mice all had significant anti-recombinant vaccine antibodies, and the BALB / c mice immunized with the recombinant vaccine spleen cells were stimulated in vitro by the recombinant vaccine Significant proliferation and produced significant IL-2. These results suggest that the candidate vaccine is immunogenic, and has some T cell stimulating ability and a certain broad-spectrum recognition ability, whether it can effectively stimulate human lymphocytes needs further study.