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目的研究曲古抑菌素A(TSA)对人胃癌细胞的增殖、凋亡及bcl-2、caspase-3 mRNA表达的影响及其意义。方法体外培养人胃癌细胞株SGC-7901,MTT法检测不同浓度TSA作用不同时间后细胞的增殖情况;流式细胞术检测不同浓度TSA作用48 h后的细胞凋亡情况;RT-PCR检测胃癌细胞中bcl-2、caspase-3 mRNA表达情况。结果不同浓度TSA作用不同时间后,随着时间延长及浓度增高,细胞的增殖抑制明显,呈现明显时间-效应关系及剂量效应关系;与阴性对照组比较,随着TSA浓度的增加,胃癌细胞SGC-7901的早期凋亡率逐渐增高,差异有统计学意义(P<0.05);与阴性对照组比较,胃癌细胞中bcl-2 mRNA随TSA浓度的增高,表达逐渐下调,而caaspase-3mRNA随TSA浓度的增高表达逐渐上调,差异有统计学意义(P<0.05)。结论 TSA对人胃癌细胞SGC-7901具有明显生长抑制作用,呈时间与剂量依赖性;能诱导人胃癌细胞SGC-7901发生早期凋亡能诱导胃癌细胞株SGC-7901细胞发生早期凋亡;TSA抑制人胃癌细胞SGC-7901增殖与诱导凋亡作用可能与bcl-2 mRNA表达下调、caspase-3mRNA表达上调有关。
Objective To study the effect and significance of trichostatin A (TSA) on proliferation, apoptosis and the expression of bcl-2 and caspase-3 mRNA in human gastric cancer cells. Methods Human gastric cancer cell line SGC-7901 was cultured in vitro. The proliferation of human gastric cancer cell line SGC-7901 was detected by MTT assay. The apoptosis of cells was detected by flow cytometry (TSA) at different concentrations of TSA for 48 h. In bcl-2, caspase-3 mRNA expression. Results After different concentrations of TSA were administered at different times, the proliferation of cells was significantly inhibited with the increase of time and concentration, showing a significant time-effect relationship and dose-response relationship. Compared with the negative control group, with the increase of TSA concentration, SGC -7901, the difference was statistically significant (P <0.05). Compared with the negative control group, the expression of bcl-2 mRNA in gastric cancer cells decreased gradually with the increase of TSA concentration, while the expression of caaspase-3 mRNA decreased with TSA Increased expression of the concentration gradually increased, the difference was statistically significant (P <0.05). Conclusion TSA can inhibit the growth of human gastric cancer cell line SGC-7901 in a dose-and time-dependent manner. Early apoptosis of human gastric cancer cell line SGC-7901 can induce early apoptosis of SGC-7901 cell line and TSA inhibition Proliferation and induction of apoptosis of human gastric cancer cell SGC-7901 may be related to down-regulation of bcl-2 mRNA and up-regulation of caspase-3 mRNA.