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收集湖北省间日疟现症病人、无症状病人及复发病人滤纸血，按ＥＬＩＳＡ法检测抗间日疟原虫环子孢子蛋白（ＣＳＰ）Ⅰ型（ＧＤＲＡＤＧＱＰＡ）、Ⅱ型（ＡＮＧＡＧＮＱＰＧ）和Ⅲ型（“Ｐ．ｖｉｖａｘ－ｌｉｋｅ”）（ＡＰＧＡＮＱＥＧＧＡＡ）重复体抗体。结果发现８３例受检血样中有３２例至少存在１型抗体，其中有Ⅰ型抗体的２７例、Ⅱ型抗体的５例、Ⅲ型抗体的１６例，包括两型混合感染的１０例和三型混合感染的４例。但只有Ⅰ型能被ＰＣＲ－探针确认。所收集的４份湖北间日疟病人全血中，虽然无１例检测到抗重复体抗体，但有３例被ＰＣＲ－探针确定为Ｉ型ＣＳＰ间日疟原虫单纯感染。本文还首次注意到间日疟原虫复发与Ⅰ型抗ＣＳＰ重复体ＩｇＧ抗体水平相关，并认为是休眠体刺激的结果。
Collect filter paper blood of patients with P. vivax, asymptomatic patients and recurrent patients in Hubei Province. ELISA was used to detect GDRADGQPA, ANGAGNQPG and Ⅲ “P. vivax-like”) (APGANQEGGAA) replicate antibody. The results showed that there were at least 32 type 1 antibodies in 32 of the 83 blood samples tested, including 27 cases of type I antibody, 5 cases of type II antibody and 16 cases of type III antibody, including 10 cases of mixed type II infection and 10 cases of tris Mixed infection in 4 cases. However, only type I can be confirmed by PCR-probe. Of the 4 collected whole blood of P. falciparum collected from Hubei Province, 3 cases were identified as simple infection of Plasmodium vivax with type I CSP by PCR-probe, although none of them detected anti-duplication antibody. We also note for the first time that P. vivax relapse correlates with the level of type I anti-CSP repeat IgG antibodies and is thought to be the result of dormant stimulation.