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目的 :了解各型乙型肝炎 (乙肝 )中HBV DNA的含量与HBs特异性免疫复合物 (HBs IC)的关系及其意义。方法 :共检测 172例血清标本。用定量PCR测HBV DNA及ELISA法测HBs IC。结果 :(1) 各型乙肝患者HBs IC阳性率和含量都明显升高 ,但HBV DNA阳性组的乙肝患者升高更为显著 ;HBs IC与HBV DNA的测定结果无明显相关 ;(2 ) HBV DNA阳性率在不同肝病有所不同 ;在HBV DNA阳性组中 ,DNA复制量在各病型无明显差异 ;(3) .HBs IC检出率在无症状携带者最低 (59% ) ;慢性乙肝轻度、中度和原发性肝癌检出率中等 (6 0 %、75%及 80 % ) ;慢性乙肝重度 ,乙肝后肝硬化及急性乙肝检出率最高 ,均为 10 0 %。结论 :在免疫应答功能正常的机体中 ,HBV DNA的扩增常伴有HBs IC的升高。推测 :HBs IC产生主要与机体的免疫功能有关 ,对HBV DNA的复制及清除有一定意义
Objective: To investigate the relationship between HBV DNA content and HBs IC in various types of hepatitis B (HBV) and its significance. Methods: A total of 172 serum samples were tested. Quantitative PCR detection of HBV DNA and ELISA assay HBs IC. Results: (1) The positive rate and content of HBs IC in all kinds of hepatitis B patients were significantly increased, but the HBV DNA positive patients had more significant increase in HBsAg level. There was no significant correlation between HBs IC and HBV DNA; (2) DNA positive rate in different liver disease is different; in HBV DNA positive group, DNA replication in the various types of no significant difference; (3) HBs IC detection rate in asymptomatic carriers the lowest (59%); chronic hepatitis B Mild, moderate and primary liver cancer detection rate was moderate (60%, 75% and 80%); chronic hepatitis B severe hepatitis B cirrhosis and acute hepatitis B highest detection rate, both 10%. CONCLUSIONS: HBV DNA amplification is often accompanied by an increase in HBs IC in a normal immune response body. Speculated that: HBs IC produced mainly with the body’s immune function, HBV DNA replication and removal of some significance