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通过对板栗种子超低温保存前后种子发芽率和脱水耐性,以及其离体胚的脯氨酸含量和丙二醛含量的测定分析,来研究板栗种子的超低温保存技术,结果表明:含水量水平是影响板栗种子发芽率以及超低温保存效果的重要因素,超低温保存时板栗种子和离体胚应进行适度脱水,超低温保存宜采用20%~25%含水量范围;采取适当脱水方式和含水量水平,能够较好地保持板栗种子的发芽率。适当的超低温保存因素的组合有利于板栗种子活力的维持、离体胚脯氨酸的诱导和减少丙二醛含量的积累。
The ultra-low temperature preservation of chestnut seeds was studied by measuring the germination rate and dehydration tolerance of chestnut seeds before and after cryopreservation, as well as their proline content and malondialdehyde content in vitro embryos. The results showed that the water content level was affected Chestnut seed germination rate and the effect of ultra-low temperature preservation of important factors, cryopreservation chestnut seeds and ex vivo embryos should be moderate dehydration, ultra-low temperature preservation should adopt the range of 20% to 25% moisture content; appropriate dehydration methods and water content levels, can compare Good to maintain the germination rate of chestnut seeds. The combination of appropriate cryopreservation factors favors the maintenance of chestnut seed vigor, the induction of proline in ex vivo embryos and the reduction of accumulation of malondialdehyde.