我国的家蚕资源丰富,利用家蚕生物反应器制备甲型流感病毒样颗粒疫苗,可促进我国桑蚕产业发展。用多基因表达家蚕生物反应器,首次构建了同时携带3个甲型H1N1流感病毒基因的重组家蚕杆状病毒,并成功在家蚕体内装配出甲型H1N1流感病毒样颗粒。为应用开发甲流感病毒全价疫苗提供一种新方法。 China’s silkworm is rich in resources, the use of silkworm bioreactor preparation of influenza A virus-like particle vaccine, can promote the development of China’s silkworm industry. With the multi-gene expression of silkworm bioreactor, the recombinant silkworm baculovirus harboring the three H1N1 influenza virus genes was constructed for the first time and the H1N1 influenza virus like particle was successfully assembled in the silkworm. It provides a new method for the application of full-price influenza A virus vaccine.