采用高效液相色谱(HPLC)、气相-电子捕获器(GC-ECD)两种不同仪器对硝酸甘油的血药浓度进行测定.结果表明,HPLC最低检测限为血中500ng/ml,日内、日间差异均小于10%,回收率大于70%.GC-ECD最低检测限为血中200pg/ml,日内、日间差异均小于10%,回收率大于70%.证明GC-ECD是可靠、稳定、重复性强的测定方法.可以指导临床医生合理应用GTN制剂,改善冠心病心绞痛患者的生存质量,减少冠心病心脏猝死的发生. The plasma concentrations of nitroglycerin were determined by high performance liquid chromatography (HPLC) and gas chromatography-electron capture (GC-ECD) instrument.The results showed that the minimum detection limit of HPLC was 500ng / ml in blood, The difference was less than 10% and the recovery was more than 70% .The lowest detection limit of GC-ECD was 200pg / ml in blood, the difference was less than 10% in day and in day, and the recovery was more than 70% .GC-ECD was proved to be reliable and stable , Repeatability of the determination of the method can guide clinicians reasonable use of GTN preparations to improve the quality of life of patients with angina pectoris and reduce the incidence of sudden cardiac death in patients with coronary heart disease.