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目的研究昆布提取物(Okam)对博莱霉素(Bleomycin)所致大鼠肺间质纤维化的抑制作用。方法Wistar大鼠经气管内注入博莱霉素诱导肺纤维化,随后每日灌胃给予Okam 25、50 mg·kg~(-1)进行干预。通过免疫组化检测胶原Ⅲ、TNF—α和血管内皮生长因子(VEGF)蛋白在大鼠肺组织的表达。结果Okam 50mg·kg~(-1)可明显降低胶原Ⅲ、TNF-α和VEGF蛋白表达;Okam 25 mg·kg~(-1)可明显降低胶原Ⅲ、VEGF蛋白表达,但TNF-α表达与模型组比较无差异。结论昆布提取物灌胃给药能减轻博莱霉素诱导的大鼠肺纤维化的程度,有可能被开发成为治疗肺纤维化的海洋药物。
OBJECTIVE: To investigate the inhibitory effect of okum extract on pulmonary interstitial fibrosis in rats induced by Bleomycin. Methods Wistar rats were intratracheally injected with bleomycin to induce pulmonary fibrosis, followed by intragastric administration of Okam 25, 50 mg·kg -1 per day. The expression of collagen III, TNF-α and vascular endothelial growth factor (VEGF) protein in rat lung tissue was detected by immunohistochemistry. RESULTS Okam 50 mg·kg -1 significantly decreased the expression of collagen III, TNF-α and VEGF protein; while Okam 25 mg·kg -1 significantly decreased the expression of collagen III and VEGF, but the expression of TNF-α was significantly decreased. There is no difference in the model group. Conclusion The intragastric administration of kelp extract can reduce the degree of bleomycin-induced pulmonary fibrosis in rats and may be developed into a marine drug for the treatment of pulmonary fibrosis.