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用精子膜抗原Ｗｅｓｔｅｒｎｂｌｏｔ分析法，对７８例生育、１９８例不孕、２２例流产者及４４例中晚期妊娠孕妇血清抗精子抗体（ａｎｔｉｓｐｅｒｍａｎｔｉｂｏｄｉｅｓ，ＡｓＡｂ）进行研究。结果发现，抗分子量８５０００、５１０００、３００００精子抗原的ＡｓＡｂ与不孕相关；抗分子量８５０００、７９０００、６９０００，５１０００精子抗原的ＡｓＡｂ与女性不孕相关；不孕女性ＡｓＡｂ种类（在Ｗｅｓｔｅｒｎｂｌｏｔ上的反应条带数）明显多于生育女性。抗分子量８５０００、７９０００、５８０００精子抗原的ＡｓＡｂ与女性ＲＳＡ相关。本研究未发现不孕组男性、流产组男性及中晚期妊娠孕妇各种分子量精子抗原的ＡｓＡｂ检出率与生育组同性别相比有显著不同。所发现的这些ＡｓＡｂ可能与不育有密切关系，需要进一步研究和证实。
The antispermantibodies (AsAb) of 78 pregnant women, 198 infertile women, 22 abortions and 44 pregnant women of middle and late pregnancy were studied by Western blot analysis of sperm membrane antigen. The results showed that AsAb anti-molecular weight of 85000, 51000, 300000 sperm antigen was associated with infertility; AsAb of anti-molecular weight 85000, 79000, 69000, and 51500 sperm antigen was associated with female infertility; AsAb species of infertile female With the number of significantly more than the birth of women. AsAbs against molecular weight 85000, 79000, 58000 sperm antigens are associated with female RSA. In this study, we found no significant difference in the detection rate of AsAbs between infertile men and abortion group as well as between male and middle-late pregnant women with different molecular weight sperm antigens. These AsAbs were found to be closely related to infertility and need further study and confirmation.