论文部分内容阅读
目的探讨胸背神经和背阔肌在脊髓及脊神经节的定位,为临床作同侧C_7神经根移位修复臂丛上干损伤提供理论依据。方法选用SD大鼠20只,随机分成2组。一组在胸背神经内注入30％HRP,另一组在背阔肌内注入30％HRP。术后48h经升主动脉灌注处死,取双侧C_4～T_2脊髓节段和脊神经节,冰冻切片后利用TMB法进行呈色反应,观察切片内HRP标记细胞的情况。结果胸背神经和背阔肌注射HRP后,在脊髓可见标记的运动神经元胞体分布在同侧C_6～C_8前角,主要分布在C_7、C_8。两组C_7的HRP标记细胞数分别为[(19．60±2．61)个,x±s,下同]和(10．90±1．27)个,C_8的HRP标记细胞数分别为(21．30±3．24)个和(11．80±1．66)个。感觉神经纤维来自于同侧C_6～C_8脊神经节,各神经节的HRP标记细胞数量相近(P>0．05)。结论背阔肌主要由C_7、C_8神经根支配,胸背神经的运动纤维主要来自C_7和C_8脊髓节段,因而临床开展同侧C_7神经根移位修复臂丛神经上干损伤从理论上是可行的。
Objective To investigate the location of the thoracodorsal nerve and latissimus dorsi in the spinal cord and the spinal ganglia and to provide a theoretical basis for the clinical repair of the upper brachial plexus injury caused by the displacement of the C_7 nerve root. Methods Twenty SD rats were randomly divided into two groups. One group injected 30% HRP into the thoracodorsal nerve and the other injected 30% HRP into the latissimus dorsi. 48 h after the ascending aorta perfusion and sacrificed, take bilateral C_4 ~ T 2 spinal cord segments and spinal ganglia, frozen section after the use of TMB color reaction, observed sections of HRP-labeled cells. Results After injection of HRP into the thoracodorsal and latissimus dorsi, the labeled motor neurons in the spinal cord were distributed in the anterior horn of C_6 ~ C_8 on the ipsilateral side, mainly distributed in C_7 and C_8. The number of HRP labeled cells of C7 in the two groups were [(19.60 ± 2.61), x ± s, the same below] and (10.90 ± 1.27), respectively. The number of HRP labeled cells in C8 were 21.30 ± 3.24) and (11.80 ± 1.66), respectively. Sensory nerve fibers from the ipsilateral C_6 ~ C_8 spinal ganglia, HRJ cell number of each ganglion similar (P> 0.05). Conclusions The latissimus dorsi muscle is dominated by C_7 and C_8 nerve roots. The movement fibers of the thoracodorsal nerve mainly come from the C_7 and C_8 spinal cord segments. Therefore, it is theoretically feasible to carry out the ipsilateral C_7 nerve root transposition to repair the brachial plexus injury. of.