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利用聚丙烯酰胺垂直平板凝胶电泳法,结合酶的染色,对水稻长白六号为细胞核供体,[野败]、[冈]、[红莲]、[滇]、[BT]为细胞质的五种同核异质不育系及其保持系,进行了酯酶、过氧化物酶的同工酶谱鉴定。结果表明,上述不同材料不同发育时期的叶片具有不同的酶谱,并出现一些特异的酶带。这些特异的酶谱和酶带,代表了不同细胞质的特点,可以作为水稻雄性不育细胞质分类的有效生化指标。同一酶带在不同材料不同发育时期表现差异,说明在同核异质不育系个体发育过程中,细胞质因子对这两种同工酶基因簇的表达起着不同的调节作用。这一结果,支持褚圻、王云侠(1984)对现有水稻雄性不育细胞质的分类。
Polyacrylamide vertical plate gel electrophoresis and enzyme staining were used to detect the cytoplasm of rice Changbai VI as the nuclear donor, [wild faeces], [Gang], [HL], [Dian], [BT] Five iso-nuclear heterologous lines and their maintainer lines were identified by isoenzyme analysis of esterase and peroxidase. The results showed that the leaves of different materials at different developmental stages had different enzyme patterns and some specific bands appeared. These specific zymograms and bands, representing different cytoplasmic characteristics, can be used as an effective biochemical indicator of the cytoplasmic classification of male sterility in rice. The same bands showed differences in different developmental stages of different materials, indicating that cytoplasmic factors play different roles in regulating the expression of these two isozymes during the development of individuals with the same heterogenic sterility. This result supports the classification of existing male sterile cytoplasms in rice by Chu and Wang (1989).