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用RT-PCR法从葡萄扇叶病毒杭州分离物(GFLV-H)基因组RNA中扩增了该病毒分离物的外壳蛋白基因cDNA片段,并克隆到质粒pGEM-T-easyVector.序列分析结果表明,该基因含有1515个核苷酸,编码504个氨基酸,其核苷酸和推导的氨基酸与GFLV法国分离物F13的同源性分别为88%和95%.目前该基因已成功地克隆到植物表达载体pBI121,经三亲交配导入到根癌农杆菌(Agrobacteriumtumefaciens)LBA4404中.
The coat protein gene cDNA fragment of the isolate was amplified by RT-PCR from the isolate of grapevine leaf isolate Hangzhou (GFLV-H) and cloned into the plasmid pGEM-T-easyVector. The sequence analysis showed that, The gene contains 1515 nucleotides and encodes 504 amino acids.The nucleotide and deduced amino acids of the gene are 88% and 95% homologous to the F13 of GFLV isolates respectively.The gene has been successfully cloned into plant expression Vector pBI121 was introduced into Agrobacterium tumefaciens LBA4404 by triparental mating.