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时间:60分钟 一、阅读下文,并回答以下各问题1~3 (20分)。 把菠菜叶放进适当的溶液中,进行研磨。 将研磨液用纱布过滤后,除去未磨碎的组织, 得到一种绿色的液体。把其倒入离心管中, 进行适当强度的离心分离,得到沉淀(沉淀A)。将此沉淀用电子显微镜进行观察时, 可发现细胞壁的碎片和(甲)膜上具有许多小 孔的环状结构。 将其上清液的绿色部分进行较强力的离心分离 时,绿色部分几乎全部沉淀(沉淀B)。用 光学显微镜观察此沉淀,发现有许多(乙)直 径为几微米的经胞器。又把几乎透明的上清 液,用更强的离心力进行分离时,可得沉淀 (沉淀C)。用电子显微镜观察此沉淀,则发 现(丙)许多直径约0.5微米的球形或短棒 形,内外包着两层膜且内膜向内折叠的组胞 器。如继续进行强力离心时,可使上部的澄清 部分中的小微粒都沉淀,此沉淀(沉淀D)其 中含有许多(丁)直径约0.02微米的致密小颗 粒和(戊)由该颗粒所附着的膜构成的细胞器。 问1:上述文章中划线处(甲)~(戊) 所示的细胞结构名称分别是什么?由下列①~ ④选出一个正确的组合。(1)。 问2:下文(1~9)是对上述沉淀(A~ D)所含有关细胞器的叙迷。试从下列①~
Duration: 60 minutes I. Read the following and answer questions 1 to 3 (20 points) below. Place the spinach leaves in a suitable solution and grind them. After polishing the slurry with gauze, the unground tissue was removed to obtain a green liquid. Pour it into a centrifuge tube and conduct centrifugation with appropriate strength to obtain a precipitate (precipitate A). When this precipitate was observed with an electron microscope, fragments of cell walls and ring structures having many small pores on the (a) membrane were found. When the green portion of the supernatant was subjected to strong centrifugal separation, almost all of the green portion precipitated (precipitate B). This precipitate was observed with an optical microscope and found to have many (b) meridians having a diameter of several micrometers. When the almost transparent supernatant is separated again with stronger centrifugal force, a precipitate (precipitate C) can be obtained. Observation of the precipitate with an electron microscope revealed that many (c) spheres or short rods having a diameter of about 0.5 μm contained two cells and were inwardly folded with the inner membrane folded inward. When the strong centrifugation is continued, the small particles in the upper clear part can be precipitated. This precipitate (precipitate D) contains a number of dense small particles (D) with a diameter of about 0.02 μm and (E) is attached by the particles. Membrane organelles. Question 1: What are the names of the cell structures shown in the lines (A) to (E) in the above article? Select the correct combination from the following 1 to 4. (1). Question 2: The following (1 to 9) is a description of the organelles involved in the above precipitation (A to D). Try from the following 1 ~