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为明确高丹草Sorghum bicolor×S.sudanense在盐碱互作胁迫下的生理响应及耐受特点,以两种中性盐NaCl和Na_2SO_4以及两种碱性盐Na_2CO_3和NaHCO_3按不同比例配成50、100、150和200mmol·L~(-1) 4个盐浓度,并且每个盐浓度分别设7.0、8.0、9.0和10.0共4个pH梯度的盐碱互作组合,研究了在盐碱互作胁迫对高丹草叶片的叶绿素荧光参数的影响。结果表明,高丹草叶片的各生理参数受高盐浓度以及溶液中各离子浓度的影响较大,其中影响较大的为CO_3~(2-)浓度和总盐浓度。低盐浓度下,不同pH对高丹草叶片叶绿素荧光参数的影响相对较小,并且低于100mmol·L~(-1)的盐浓度下高丹草叶片PSⅡ反应中心的光化学活性无明显影响,即高丹草具有一定的抗盐碱性能力。但在高盐高pH条件下,高丹草叶片PSⅡ光化学效率的降低,并且此时随着pH的增加,高丹草叶片的PSⅡ反应中心活性降低幅度增大。高丹草在一定碱性盐浓度范围内可以通过提高非化学淬灭系数(NPQ)及时耗散过剩的光能,但在高盐浓度下高丹草叶片通过NPQ来耗散过剩光能的保护能力下降,并且高pH下降低幅度更为显著。盐和pH对高丹草叶片的各生理参数影响过程中存在明显的交互作用,并且随着盐浓度的增加,交互作用逐渐变大,即低盐浓度下,受pH的影响相对较小,但随着盐浓度的增加,pH的影响变大。高丹草叶片具有一定的耐盐碱能力,但在盐浓度较高地区推广高丹草要注意碱化度的影响。
In order to clarify the physiological response and tolerance characteristics of Sorghum bicolor × S.sudanense under salt-alkali interaction, two neutral salts NaCl and Na_2SO_4 and two basic salts Na_2CO_3 and NaHCO_3 were formulated into 50 , 100, 150 and 200 mmol·L -1, respectively, and four salinity-alkaline interaction combinations of pH gradient of 7.0, 8.0, 9.0 and 10.0 for each salt concentration were studied. Effects of Stress on Chlorophyll Fluorescence Parameters in Leaves of Sorghum. The results showed that the physiological parameters of Sorghum sudanense leaves were greatly affected by the high salt concentration and the concentration of ions in the solution, and the concentrations of CO_3 ~ (2-) and total salt were significant. Under low salt concentration, the effect of different pH on the chlorophyll fluorescence parameters of Sorghumgrass leaves was relatively small, and the photochemical activities of PSⅡ reaction center of Sorghumgrass leaves had no significant effect when the salt concentration was lower than100mmol·L -1, That Dan Dao grass has some anti-salty alkaline ability. However, under high salt and high pH conditions, the photochemical efficiency of PSⅡ decreased in the leaves of Sorghum sudanense, and the PSⅡ reaction center activity decreased with the increase of pH at this time. Sorghum sudanera could dissipate excess light energy in time by increasing non-chemical quenching coefficient (NPQ) within a certain range of basic salt concentration. However, Sorghum sudanense leaves could dissipate excess light energy through NPQ at high salinity The ability to decline, and the high pH down more significantly. There was a significant interaction between salt and pH on the physiological parameters of Sorghum sudanense leaves, and the interactions became larger with the increase of salt concentration, that is, the effect of pH was relatively small under low salt concentration As the salt concentration increases, the effect of pH becomes greater. Sorghum leaf has certain ability of resistance to salt and alkali, but in the area of higher salt concentration to promote Sorghum degree of alkalinity should pay attention to the impact.