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目的:通过测定错配修复基因hMLH1在散发性结直肠癌(CRC)组织中的蛋白表达变化,分析其在散发性CRC发生机制中所起的作用,并评估其可能的临床意义。方法:采用PV-6000二步法免疫组化检测技术对60例散发性CRC石蜡切片进行hMLH1表达的检测。结果:60例散发性CRChMLH1缺失表达11例(18.3%,11/60),hM-LH1缺失表达与患者年龄、肿瘤部位、组织学类型和分化程度密切相关(P<0.05或P<0.01),而与患者性别、肿瘤大体类型、肿块大小、浸润深度、淋巴结及远处转移与否和患者的Dukes分期均无显著相关性(P>0.05)。结论:一定比例的散发性CRC中存在MMR基因的缺陷,并具有相对特殊的临床病理特征。
OBJECTIVE: To determine the possible role of hMLH1 in the pathogenesis of sporadic CRC by measuring the protein expression of mismatch repair gene hMLH1 in sporadic colorectal cancer (CRC) and to evaluate its possible clinical significance. Methods: Sixty cases of sporadic CRC paraffin sections were examined for hMLH1 expression by PV-6000 two-step immunohistochemistry. Results: There were 11 cases (18.3%, 11/60) in 60 cases of sporadic CRChMLH1 deletion. The expression of hM-LH1 was closely related to the age, tumor location, histological type and differentiation (P <0.05 or P <0.01) However, there was no significant correlation between the Dukes staging and gender, type of tumor, size of tumor, depth of invasion, lymph node and distant metastasis and Dukes stage (P> 0.05). Conclusion: A certain proportion of sporadic CRC exists the defects of MMR gene and has a relatively special clinicopathological features.