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目的:观察益气解毒法对胃癌细胞增殖活性的干预作用。方法:空白血清、复方环磷酰胺含药血清、不同剂量(低剂量、中剂量和高剂量)益气解毒法含药血清,分别与人胃癌细胞SGC-7901共培养24、48、72、96 h,MTT法测其对胃癌细胞增殖的抑制率;流式细胞技术测含药血清与人胃癌细胞SGC-7901共培养48 h后的细胞周期。结果:益气解毒法含药血清依照剂量从低到高的顺序,其吸光度(OD)值依次降低并与刺激时长负相关,抑制率(IR)依次升高并与刺激时长正相关;在24~72 h抑制强度随时间逐渐增强,72~96 h抑制强度略有降低。益气解毒法低、中、高剂量各组含药血清在分别刺激胃癌SGC-7901细胞48 h后,其G0/G1期细胞比率显著增加(P<0.05),并与益气解毒法的剂量正相关;S期和G2/M期细胞比率均显著减少(P<0.05),并与益气解毒法的剂量负相关。结论:益气解毒法对胃癌细胞增殖有抑制作用。
Objective: To observe the intervention of Yiqi Jiedu Method on the proliferation of gastric cancer cells. Methods: Serum containing serum, compound cyclophosphamide drug-containing serum, different doses (low dose, medium dose and high dose) Yiqi detoxification drug-containing serum were co-cultured with human gastric cancer cells SGC-7901 24,48,72,96 h, the inhibition rate of gastric cancer cell proliferation was measured by MTT method. The cell cycle of the gastric cancer cell SGC-7901 co-cultured with SGC-7901 for 48 h was measured by flow cytometry. Result: The OD value of the serum containing Yiqi Jiedu drug decreased in sequence from low to high and negatively correlated with the duration of stimulation. The inhibition rate (IR) increased in turn and positively correlated with the duration of stimulation. At 24 The inhibitory intensity of ~ 72 h gradually increased with time, and the inhibitory intensity of 72 ~ 96 h decreased slightly. Gastric cancer SGC-7901 cells were stimulated by Qi, detoxification at low, medium and high doses for 48 hours, respectively. The percentage of cells in G0 / G1 phase increased significantly (P <0.05) (P <0.05). The ratio of cells in S phase and G2 / M phase was significantly decreased (P <0.05), and negatively correlated with the dosage of Yiqi detoxification method. Conclusion: Yiqi Jiedu method can inhibit the proliferation of gastric cancer cells.