论文部分内容阅读
选用纤维比强度形成存在温度敏感性差异的两个棉花品种(科棉1号:温度弱敏感型品种,苏棉15:温度敏感型品种)为材料,于2006—2007年在江苏南京设置大田分期播种试验,使棉纤维发育处于不同的温度条件,研究低温对棉纤维发育相关酶(蔗糖酶、蔗糖合成酶、磷酸蔗糖合成酶、β-1,3-葡聚糖酶)活性及相应基因表达的影响.结果表明:由晚播造成的低温(棉纤维发育期日均最低温分别为21.1、20.5和18.1℃)影响了纤维发育相关酶的活性变化,从而影响了棉纤维素累积和纤维比强度的形成.低温提高了纤维中蔗糖酶和β-1,3-葡聚糖酶的活性,降低了蔗糖合成酶和磷酸蔗糖合成酶的活性.低温使Expansin、蔗糖合成酶基因的高表达时间延长,β-1,3-葡聚糖酶基因的表达峰值出现时间延迟,且表达量降低.两个棉花品种的纤维素合成相关酶对低温的响应存在差异,温度敏感型品种(苏棉15)的酶活性的变化幅度明显高于温度弱敏感型品种(科棉1号),可能是导致不同棉花品种纤维比强度形成存在温度敏感性差异的主要原因.
Two cotton cultivars with different temperature sensitivity (Kemian No.1: temperature-sensitive and Su-mian15: temperature-sensitive) were selected as the materials for their specific fiber strength. In 2006-2007, Daejeon staging was set up in Nanjing, Jiangsu Province Sowing experiment, the cotton fiber development at different temperature conditions, low temperature on cotton fiber development related enzymes (sucrase, sucrose synthase, sucrose phosphate synthase, β-1,3-glucanase activity and the corresponding gene expression The results showed that the low temperature induced by late sowing (the daily average minimum temperature of cotton fiber was 21.1, 20.5 and 18.1 ℃ respectively) affected the activity of fiber-related enzymes, which affected the accumulation of cellulose and fiber specific strength Low temperature increased the activity of sucrase and β-1, 3-glucanase in fiber and decreased the activities of sucrose synthase and sucrose synthase.Low temperature prolonged the expansin and sucrose synthase gene expression time , the expression peak of β-1, 3-glucanase gene appeared delayed, and the expression level decreased.The response of cellulose synthesis related enzymes of two cotton varieties to low temperature was different, the temperature-sensitive variety (Sumian 15) of Activity significantly higher than the variation width of temperature sensitive cultivars weak (Kemian 1), may be the main cause of temperature sensitivity difference of the presence of different cultivars of cotton fiber strength formation.