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目的:评价诺氟沙星对烧烫伤创面常见致病菌的抑菌、杀菌作用。方法:用浊度法测定诺氟沙星对金黄色葡萄球菌(ATCC6538)、大肠杆菌(ATCC8099)和铜绿假单胞菌[CMCC(B)10110]的最小抑菌浓度(MIC)和最小杀菌浓度(MBC),实验平行3次,观察诺氟沙星培养液的浊度。结果:诺氟沙星对金黄色葡萄球菌的MIC为0.15μg·ml~(-1),MBC为0.25μg·ml~(-1);对大肠杆菌的MIC为0.25μg·ml~(-1),MBC为5.0μg·ml~(-1);对铜绿假单胞菌的MIC为4.57μg·ml~(-1),MBC为14.0μg·ml~(-1)。结论:诺氟沙星在体外对烧烫伤创面常见致病菌均具有较强的抗菌作用,可为临床创面用药剂量的筛选提供一些依据。
Objective: To evaluate the bacteriostatic and bactericidal effects of norfloxacin on common pathogenic bacteria in burn wounds. Methods: The minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MIC) of norfloxacin against Staphylococcus aureus (ATCC6538), Escherichia coli (ATCC8099) and Pseudomonas aeruginosa [CMCC (MBC). The experiment was performed in parallel for 3 times to observe the turbidity of norfloxacin medium. Results: The MICs of norfloxacin against Staphylococcus aureus were 0.15 μg · ml -1 and MBC was 0.25 μg · ml -1. The MIC of Escherichia coli was 0.25 μg · ml -1 ), The MBC was 5.0μg · ml -1; the MIC of Pseudomonas aeruginosa was 4.57μg · ml -1, MBC was 14.0μg · ml -1. CONCLUSION: Norfloxacin has strong antibacterial activity against common pathogenic bacteria in burned wounds in vitro, which may provide some basis for the screening of clinical wound dosage.