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目的:建立发育期慢性癫痫大鼠模型,观察海马主穹窿蛋白(MVP)的表达及左乙拉西坦(LEV)对其表达的影响。方法:腹腔注射红藻氨酸(KA)1 mg/kg(浓度0.5 mg/ml),建立大鼠慢性癫痫模型,注射后连续观察8 h,癫痫发作达5级以上并出现癫痫持续状态的大鼠,且两周后出现自发性反复惊厥发作者视为模型成功。将造模成功后的40只大鼠随机分为未治疗组(KA组)20只,左乙拉西坦治疗组(KA+LEV组)20只,另取40只大鼠腹腔注射生理盐水,并分为NS组20只、NS+LEV组20只。用药组均于癫痫自发性反复发作后开始用药,疗程为6周,然后将所有大鼠断头取脑,用免疫组化及RT-PCR法测定大鼠海马内MVP及其mRNA的表达。结果:(1)大鼠海马MVP的阳性细胞计数与MVP的mRNA表达趋势相一致。(2)NS组、NS+LEV组大鼠海马有少量MVP阳性细胞及mRNA表达,NS+LEV组MVP阳性细胞数及mRNA的含量与NS组相比差异无统计学意义(P>0.05);KA组MVP阳性细胞计数及mRNA的含量与NS组相比显著增高(P<0.05);KA+LEV组与KA组相比,MVP的阳性细胞及mRNA含量减少(P<0.05)。结论:MVP可能参与慢性癫痫耐药的发生,LEV可以控制大鼠痫性发作,并下调MVP的表达。
OBJECTIVE: To establish a chronic epilepsy rat model of development and observe the effects of levodocisol (LEV) on the expression of vWM in the hippocampus. Methods: Chronic epilepsy model was established by intraperitoneal injection of kainic acid (KA) 1 mg / kg (concentration 0.5 mg / ml) for 8 hours. The epileptic seizures up to level 5 and the status of epileptic seizures were large Rats, and spontaneous recurrent seizures occurred two weeks later were considered model success. Forty rats were randomly divided into untreated group (KA group) 20, levetiracetam group (KA + LEV group) 20, the other 40 rats were injected intraperitoneally saline, And divided into NS group 20, NS + LEV group 20. The medication group was started spontaneous recurrent seizures after medication for 6 weeks, and then all rats decapitated brain, immunohistochemistry and RT-PCR method to detect the expression of MVP and its mRNA in hippocampus of rats. Results: (1) The positive cell number of hippocampal MVP in rats was consistent with the trend of mRNA expression of MVP. (2) There was a small amount of MVP positive cells and mRNA expression in NS and NS + LEV groups. There was no significant difference in the number of MVP positive cells and mRNA between NS + LEV group and NS group (P> 0.05). Compared with KA group, the number of MVP positive cells and mRNA in KA + LEV group were significantly decreased (P <0.05). Conclusion: MVP may be involved in the development of chronic epilepsy. LEV may control seizure in rats and down-regulate the expression of MVP.