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为了比较农业生产型林业中的木聚糖,使用木聚糖RmXyn10A的两种变体,只有源自海洋红嗜热盐菌的全长酶和催化模块,对硬木(桦木)木聚糖和谷物(黑麦)阿拉伯木聚糖进行水解。用含有水解产物的低聚木糖(XOS)作为碳源,培养4种精选的细菌菌种,结果显示了短乳杆菌DSMZ 1264和青春双歧杆菌ATCC 15703出现选择性生长。这两种菌株被证实可利用低聚木糖组分(DP 2-5);而来自黑麦阿拉伯木聚糖水解产物的假定阿拉伯低聚木糖仅能被青春双歧杆菌利用。大肠杆菌不能生长,但能在单糖阿拉伯糖和木糖上生长。研究还表明,小片球菌(Pediococcus parvulus)2.6株既不能利用木糖,也不能利用低聚木糖进行生长。总之,RmXyn10A或其催化模块证实可用于硬木木聚糖和谷物阿拉伯木聚糖的高温水解,产生XOS,因此能在功能性食品中充当益生元。
In order to compare xylan in agricultural production forestry, two variants of xylan RmXyn10A were used, only the full-length enzymes and catalytic modules derived from H. thermophila bacteria, the use of hardwood (birch) xylans and cereals (Rye) arabinoxylan for hydrolysis. Four selected bacteria strains were cultured with xylo-oligosaccharides (XOS) containing hydrolyzate as the carbon source and showed that the selective growth of L. brevis DSMZ 1264 and B. adolescentis ATCC 15703 occurred. Both strains were shown to utilize the xylooligosaccharides component (DP 2-5); whereas putative arabinoxylan from rye arabinoxylan hydrolyzate can only be utilized by Bifidobacterium adolescentis. E. coli can not grow but can grow on the monosaccharides arabinose and xylose. The study also showed that 2.6 strains of Pediococcus parvulus neither utilize xylose nor grow with xylose. In summary, RmXyn10A or its catalytic module has been shown to be useful as a prebiotic in functional foods due to the high temperature hydrolysis that can be applied to hardwood xylan and cereal arabinoxylan to produce XOS.