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目的:研究手机900MHz射频磁场对小鼠皮层神经元动作电位的影响。方法:低发射功率为398.1 mW、频率为900 MHz的射频磁场照射急性分离小鼠脑皮层神经元。采用膜片钳技术,观察对照组和暴露组的Na+通道电流I Na、瞬时外向K+通道电流I A和延迟整流K+通道电流I K。结果:900 MHz磁场暴露对神经元离子通道有明显影响:(1)Na+通道激活电位向超极化方向移动,钠电流峰值增大,激活和失活曲线向超极化方向移动;(2)瞬时外向钾通道的激活过程受到抑制;(3)延迟整流钾电流I K受到抑制,且呈时间依赖性。结论:低功率900 MHz射频磁场暴露影响离子通道的I-V特性和动力学特性,神经元动作电位发放阈值变小,动作电位发放频率变缓,改变了动作电位的形状和时程,影响了神经细胞电传导特性,对脑神经元的生理功能产生了一定的影响。
Objective: To study the effect of cell phone 900MHz RF magnetic field on the action potential of cortical neurons in mice. METHODS: Acutely isolated cortical neurons of mice were irradiated by RF magnetic field with a low transmit power of 398.1 mW and a frequency of 900 MHz. Patch-clamp technique was used to observe the Na + channel current I Na, the transient outward K + channel current I A and the delayed rectifier K + channel current I K in the control and exposure groups. RESULTS: Magnetic field exposure at 900 MHz had a significant effect on neuronal ion channels: (1) Na + channel activation potentials shifted toward hyperpolarization and sodium currents peaked, and the activation and inactivation curves shifted toward hyperpolarization; (2) Transient outward potassium channel activation was inhibited; (3) delayed rectifier potassium current IK was inhibited, and in a time-dependent manner. CONCLUSION: The low-power 900 MHz RF magnetic field exposure affects the IV characteristics and dynamics of ion channels. The release threshold of action potentials of neurons decreases and the action potential distribution frequency becomes slower, which changes the shape and duration of action potentials and affects the changes of nerve cells Electrical conductivity, the physiological function of brain neurons had a certain impact.