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本文利用ＲＡＰＤ分子标记技术从ＤＮＡ分子水平上探测了大青场（ｐｌｐｕｌｕｓｕｓｓｕｒｉｅｎｓｉｓＫｏｍ．）天然群体的遗传结构和分化程度。结果得出：用１４个随机寡核苷酸引物共产生１８０个扩增片段，扩增片断在２１１ｂｐ至１６３６ｂｐ之间。Ｓｈａｎｎｏｎ表型多样度（ＨＯ）估测值在群体间变动范围为０．２７１至０．３９２，平均为０．３１０。对分子水平变异分为群体间和群体内两部分进行分析，群体间分量占总变异的６２．３％，群体内只占３７．７％。不同引物在群体内探测能力也各不相同，ＣＨｌ－ｌ引物探测多样度（ＨＯ）最高（０．５４０），而２１１６引物最低（０．１５１）。
In this paper, RAPD molecular marker technique was used to detect the genetic structure and differentiation of natural population of Pichia vulgaris (Kom.) From the DNA molecular level. The results showed that a total of 180 amplified fragments were generated with 14 random oligonucleotide primers and the amplified fragments ranged from 211bp to 1636bp. The estimated Shannon’s phenotypic diversity (HO) ranged from 0.271 to 0.392 in the population with an average of 0.310. The molecular level variation was divided into two groups: intra-group and inter-group, accounting for 62.3% of the total variation and only 37.7% of the population. The ability of different primers to detect in different populations was also different, with the highest detection index (0.540) for CHl-1 primers and the lowest (0.151) for 2116 primers.