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为了解尿液单个核细胞(UMC)测定在IgA肾病(IgAN)中的临床意义,采用流式细胞仪对49例患者的尿液进行分析。 一、资料和方法 选择经肾活检诊断IgAN患者男31例,女18例,年龄14～43岁;40例健康人做为对照。留取尿液50ml,1500r/min离心10分钟,PBS中洗涤3 次,制成悬液;每个小管中加入悬液50μl,加10μl荧光标记的单个核细胞单克隆抗体,混匀,4℃孵育30分钟,PBS洗涤2次后加入溶血素1ml,加150μg固定液,洗涤3次后待测;少量肾组织在研磨后按照上述方法制成肾组织单个核细胞悬液。采用Bacton Dickenson公
In order to understand the clinical significance of urine mononuclear cells (UMC) assay in IgA nephropathy (IgAN), 49 patients were analyzed for urine using flow cytometry. First, the data and methods selected by renal biopsy IgAN patients 31 males and 18 females, aged 14 to 43 years; 40 healthy people as a control. 50ml of urine was collected, centrifuged at 1500r / min for 10 minutes, and washed in PBS three times to prepare a suspension. Each tube was added with 50μl of suspension and 10μl fluorescently labeled mononuclear cell monoclonal antibody was added and mixed at 4 ℃ Incubated for 30 minutes, washed twice with PBS after adding hemolysin 1ml, add 150μg fixative, washed three times the test; a small amount of kidney tissue after grinding in accordance with the above method to make a renal cell suspension of mononuclear cells. Bacton Dickenson male