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目的:探讨海南五指山裸花紫珠通过Wnt/β-catenin信号通路的抗肠癌作用及机制。方法:采用集落形成实验检测海南五指山裸花紫珠对肠癌细胞增殖的影响；采用HE染色法检测海南五指山裸花紫珠对肠癌细胞集落生长的影响；采用激光共聚焦显微镜检测海南五指山裸花紫珠对肠癌细胞IFITM1跨膜蛋白的影响；采用免疫细胞化学法检测海南五指山裸花紫珠对肠癌细胞抑癌蛋白的影响；采用Western Blot检测Wnt/β-catenin信号通路相关蛋白表达量。结果:海南五指山裸花紫珠浓度的增加可导致SW480和SW620细胞集落数的下调和抑制率的上调，并且呈剂量依赖关系；海南五指山裸花紫珠组（Lhzz组）SW480和SW620细胞相较对照组（con组）集落体积缩小，细胞形态较模糊，集落细胞数明显减少；Lhzz组SW480和SW620细胞相较con组IFITM1跨膜蛋白表达绿色荧光强度减弱，集落生成数减少、体积缩小，胞核固缩且形态不规则；Lhzz组的SW480细胞抑癌蛋白DCC表达水平相较con组显著上调（2.13 ± 0.33比1.05 ± 0.16，n t＝3.542，n P<0.05）；Lhzz组的SW620细胞抑癌蛋白DCC表达水平相较con组显著上调（1.89 ± 0.26比0.96 ± 0.12，n t＝3.466，n P<0.05）；Lhzz组的SW480细胞Cyclin D1表达水平相较con组显著上调（0.64 ± 0.11比0.38 ± 0.05，n t＝3.154，n P<0.05）；Lhzz组的SW620细胞Cyclin D1表达水平相较con组显著上调（0.52 ± 0.09比0.24 ± 0.03，n t＝3.143，n P<0.05）；且Lhzz组的SW480和SW620细胞β-catenin表达水平相较con组显著上调（n P<0.05）。n 结论:海南五指山裸花紫珠可以通过激活Wnt/β-catenin信号通路实现对肠癌细胞增殖生长的抑制作用。“,”Objective:To investigate the anti-intestinal effect and mechanism of Wnt/β-catenin signaling pathway in Wuzhishan CallicarpanudifloraHook.etArn in Hainan Province.Methods:The colony formation assay was used to detect the effect of Wuzhishan CallicarpanudifloraHook.etArn on the proliferation of intestinal cancer cells in Hainan Province. The effect of Wuzhishan CallicarpanudifloraHook. etArn on the colony growth of intestinal cancer cells was detected by HE staining. The effect of Wuzhishan CallicarpanudifloraHook.etArn on transmembrane protein of intestinal cancer cell line IFITM1 was detected by laser confocal microscopy. Immunocytochemistry was used to detect the effect of Wuzhishan CallicarpanudifloraHook.etArn on the tumor suppressor protein of intestinal cancer cells. Western Blot was used to detect the expression of Wnt/β-catenin signaling pathway-related proteins.Results:With the increase of Wuzhishan CallicarpanudifloraHook.etArn in Hainan Province concentration, the colony number of SW480 and SW620 cells was down-regulated and the inhibition rate was up-regulated in a dose-dependent manner. Compared with that in control group(con group), the colony size of SW480 and SW620 cells in group Wuzhishan CallicarpanudifloraHook.etArn in Hainan Province group (Lhzz group) decreased, the cell morphology was blurred and the number of colony cells decreased significantly. Compared with that in con group, the green fluorescence intensity of IFITM1 transmembrane protein expression in SW480 and SW620 cells in Lhzz group decreased, the number of colony formation decreased, the volume decreased, and the nucleus showed pyknosis and irregular shape. The expression of tumor suppressor protein DCC in SW480 cells in Lhzz group was significantly higher than that in con group (2.13 ± 0.33 vs. 1.05 ± 0.16, n t = 3.542, n P < 0.05). The expression level of tumor suppressor protein DCC in SW620 cells in con group was significantly higher than that in con group (1.89 ± 0.26 vs. 0.96 ± 0.12, n t = 3.466, n P < 0.05). The expression level of oncoprotein DCC in SW620 cells in con group was significantly higher than that in con group (0.64 ± 0.11 vs. 0.38 ± 0.05, n t = 3.154, n P < 0.05), and the expression level of tumor suppressor protein in SW620 cells in con group was significantly higher than that in con group (0.52 ± 0.09 vs. 0.24 ± 0.03, n t = 3.143, n P < 0.05), and the expression level of β-catenin in SW480 and SW620 cells in Lhzz group was significantly higher than that in con group ( n P < 0.05).n Conclusions:Wuzhishan CallicarpanudifloraHook.etArn in Hainan Province can inhibit the proliferation of intestinal cancer cells by activating Wnt/β-catenin signaling pathway.