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在1.2分辨率修正了三方二锌猪胰岛素的晶体结构。采用将广泛的立体化学观测量并入最小二乘方程加以制约的倒易空间修正方法。在对蛋白质非氢原子和氢原子、水及溶剂相分别进行各向同性温度因子的修正和校正之后,对胰岛素二体的全部非氢原子进行了两轮各向异性温度因子的修正.对于1.2范围内的20005个大于1σ的独立反射的最终R因子是0.128,与理想共价键长的均方根偏差是0.021。在最终的电子密度图上有清晰的原子水平的密度表现,硫原子的电子密度的各向异性分布表现明显。经各向异性温度因子修正后,电子密度的拟合情况有明显改善。
The crystal structure of the tripartite Zinc porcine insulin was corrected at 1.2 resolution. A reciprocal space correction method that incorporates a wide range of stereochemical observations into the least square equation is used. Two rounds of anisotropic temperature factors were corrected for all non-hydrogen atoms of the insulin doubles after the isotropy temperature factors were corrected and corrected for non-hydrogen atoms and hydrogen atoms, water and solvent, respectively. For 1.2 The final R factor for the 20005 independent reflections above 1 sigma in the range is 0.128, and the root mean square deviation from the ideal covalent bond length is 0.021. In the final electron density map has a clear atomic level of density performance, sulfur atom electron density distribution of the anisotropy obvious. After the correction of the anisotropic temperature factor, the fitting of electron density has obviously improved.