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目的 构建和表达旋毛虫重组质粒。方法 PCR法在旋毛虫抗原基因Ts87两端加上合适的酶切位点和KOZAK序列,与真核表达载体pcDNA3.1(+)连接,构建pcDNA3.1/Ts87重组质粒。采用Lipofectamine介导的细胞外源DNA转染法,把重组质粒导入COS7细胞。分别用肌肉注射和基因枪免疫小鼠。结果和结论 成功构建pcD-NA3.1/Ts87重组质粒,并在COS7细胞和BALB/c小鼠体内表达。
Objective To construct and express Trichinella spiralis recombinant plasmid. METHODS: The recombinant plasmid pcDNA3.1 / Ts87 was constructed by adding the appropriate restriction enzyme sites and KOZAK sequence to the Trichinella antigens gene Ts87 at both ends of the plasmid. Recombinant plasmids were introduced into COS7 cells using Lipofectamine-mediated exogenous DNA transfection. Mice were immunized with intramuscular and gene gun respectively. Results and Conclusion The pcD-NA3.1 / Ts87 recombinant plasmid was successfully constructed and expressed in COS7 cells and BALB / c mice.