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用抗小鼠CD3 单抗刺激幼龄小鼠胸腺细胞 ,培养不同时间后 ,检测小鼠胸腺细胞的凋亡情况。结果表明 ,胸腺细胞呈现了凋亡的典型形态学改变 ,流式细胞仪检测可见凋亡细胞特有的AP峰 ,CD3 单抗刺激未成熟胸腺细胞可以通过内源性的凋亡途径引起细胞死亡。
Thymocytes of young mice were stimulated with anti-mouse CD3 monoclonal antibody, and the apoptosis of mouse thymocytes was detected after different time. The results showed that thymocytes showed the typical morphological changes of apoptosis. Flow cytometry revealed AP peak specific to apoptotic cells. CD3 mAb stimulated immature thymocytes to cause cell death via endogenous apoptotic pathway.