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背景:缺血缺氧性脑病(hypoxic-ischemicbraindamage,HIBD)是引起脑性瘫痪的常见病,目前对其治疗尚未有特效的方法。目的:探讨氦氖激光穴位照射与电针穴位刺激两种疗法对新生大鼠脑缺血缺氧后海马神经元存活、发育及其脑源性神经营养因子(brain-derivedneurotrophicfactor,BDNF)、胆碱乙酰基转移酶(cholineacetyltransferase,ChAT)表达的作用,比较与分析两种疗法作用的差异,探讨治疗HIBD的新方法。设计:完全随机设计,实验对照研究。地点与材料:研究的地点为第二军医大学和郑州大学解剖教研室。材料为58只7d龄Wistar大鼠(购自河南省实验动物中心),体质量12～15g,雌雄不拘。干预:随机分为4组:对照组、缺血缺氧组、缺血缺氧后氦氖激光穴位照射组(简称激光组)和缺血缺氧后电针穴位刺激组(简称电针组)。缺血缺氧组～电针组动物参照于晓虹的半球性脑缺血缺氧实验模型法制作左半球缺血缺氧模型,激光组-电针组均选择“大椎”和“百会”穴位分别给予激光照射与电针刺激,22d后取脑作组织切片,HE、Nissl染色以及BDNF和ChAT免疫组织化学染色。主要观察指标:光镜下观察神经元及其尼氏体,检测灰度值与记数ChAT和BDNF阳性细胞以及统计学处理。结果:①对照组海马神经元结构清晰,胞浆内充满深蓝色尼氏体(灰度值122.90±12.
BACKGROUND Hypoxic-ischemic brain injury (HIBD) is a common disease that causes cerebral palsy. At present, there is no specific treatment for HIBD. OBJECTIVE: To investigate the effects of He-Ne laser acupoint irradiation and electroacupuncture (EA) acupoint stimulation on the survival and development of hippocampal neurons and the expression of brain-derived neurotrophic factor (BDNF), choline Acetyltransferase (ChAT) expression in the role of comparison and analysis of the difference between the two therapies to explore the new method of treatment of HIBD. Design: completely random design, experimental control study. Location and Materials: The research sites were the Second Military Medical University and the Department of Anatomy, Zhengzhou University. The material was 58 Wistar rats of 7 days old (purchased from Experimental Animal Center of Henan Province) with body weight of 12-15g, both male and female. Interventions were randomly divided into 4 groups: control group, ischemia-hypoxia group, He-Ne laser acupoint irradiation group (referred to as laser group) and EA acupoint stimulation group (EA group) . The models of ischemia-hypoxia and electro-acupuncture in the left hemisphere model of ischemia and hypoxia were made according to the experimental model of hemispheric cerebral ischemia and hypoxia in Xiaohong. The “Dazhui” and “Baihui” Acupoints were given laser irradiation and electroacupuncture stimulation. Brain tissue sections were obtained after 22 days, HE, Nissl staining and BDNF and ChAT immunohistochemical staining. MAIN OUTCOME MEASURES: Neurons and their Nissl bodies were observed under light microscope. ChAT and BDNF positive cells were detected by gray value and count, and statistical analysis was performed. Results: ① The hippocampal neurons in the control group had a clear structure with cytoplasm filled with deep blue Nissl (gray value 122.90 ± 12.