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目的探讨导流杂交基因芯片技术和第二代杂交捕获法检测宫颈液标本中人乳头状瘤病毒(HPV)感染的一致性,评价导流杂交基因芯片技术的临床应用价值。方法采用导流杂交基因芯片技术和第二代杂交捕获法对356例疑似HPV感染的标本进行基因诊断,对检测结果不一致的标本进行DNA测序,根据测序结果进行HPV分型,比较这两种方法对13种高危型HPV检测结果的符合情况。结果导流杂交基因芯片技术检测13种高危型HPV的检出率78.9%(281/356),其中单一感染217例,多重感染64例;第二代杂交捕获法检测结果阳性280例;两种方法检测13种高危型HPV的总符合率92.6%,总Kappa指数(KI)0.891,二者有很好的一致性。结论导流杂交基因芯片技术适合更临床筛查HPV感染及对HPV进行基因分型。
Objective To investigate the consistency of flow-through hybridization (PCR) microarray and the second generation hybridization capture method for detection of human papillomavirus (HPV) infection in cervical fluid specimens and to evaluate the clinical value of flow-through hybridization gene chip technology. Methods 356 cases of suspected HPV infection were genotyped by flow-through hybridization microarray and second-generation hybridization and DNA sequencing was performed on specimens with inconsistent test results. HPV typing was performed according to the sequencing results. The two methods were compared 13 kinds of high-risk HPV test results in line with the situation. Results The detection rate of 13 kinds of high-risk HPV was 78.9% (281/356) by flow-through hybridization gene chip technique, including 217 cases of single infection and 64 cases of multiple infection. The second-generation hybridization detection method was positive of 280 cases. Methods The total coincidence rate and the total Kappa index (KI) of 13 high-risk HPVs were 92.6% and 0.891, respectively. The two methods were in good agreement. Conclusion Diversion gene chip technology is suitable for more clinical screening of HPV infection and HPV genotyping.