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由于细胞遗传学研究的进展,苯对染色体损伤的研究相继展开,并已得到广泛的应用。微核作为染色体损伤的指标之一,具有方法简便、结果快速、经济、适用等优点。对苯中毒大鼠进行骨髓细胞微核率的观察,以探求苯对骨髓细胞染色体的损伤获得进一步认识,为苯的作用机理及临床提供遗传学方面的参考数据。一、对象与方法 (一)亚慢性苯中毒大鼠模型的制备选择健康成年Wistar雌性大鼠25只,体重在210～280克之间,分为二组,即注射组6只,吸入组19只。注射组大鼠系将纯苯用茶油制成混悬液,每次注射苯量为264mg/kg,每周连续六天。吸入组用1M~3中毒柜作静式吸入染毒,依柜内含苯不同分为三组,每天使用纯苯量甲组为2.5ml,吸毒6小时,乙组为15.0ml,吸毒3小时,丙组为10.0～15.0ml,吸毒3～6小
Due to the advances in cytogenetics research, the study of chromosomal damage by benzene has been carried out one after another and has been widely used. As one of the indicators of chromosome damage, micronucleus has the advantages of simple method, rapid results, economy, and application. To observe the micronuclear rate of bone marrow cells in benzene poisoned rats, and to seek further understanding of benzene damage to bone marrow cells chromosomes, and to provide reference data for the mechanism of benzene and clinical aspects of genetics. I. Objects and Methods (I) Preparation of Subchronic Benzene Poisoning Rat Model 25 healthy adult female Wistar rats were selected, weighing between 210 and 280 grams and divided into two groups, ie, 6 injection groups and 19 inhalation groups. . In the injection group, pure benzene was made into a suspension with tea oil. The amount of benzene per injection was 264 mg/kg for six consecutive days per week. The inhalation group used 1M~3 poisoning cabinet for static inhalation and was divided into three groups according to the benzene content in the cabinet. The amount of pure benzene in each group was 2.5ml per day, 6 hours for drug use, 15.0ml for group B, and 3 hours for drug use. , C group is 10.0 to 15.0ml, drug use 3 to 6 small