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目的了解广东地区临床患者和献血者HCV基因型(亚型)分布。方法收集广东地区临床患者HCV RNA阳性标本191(人)份、无偿献血者HCV RNA阳性标本222(人)份,采用逆转录巢式PCR对HCV E1基因扩增并测序;应用MEGA5软件构建分子进化树并对其做基因分型。采用SPSS16.0软件对2组人群中不同基因亚型的分布做比较。结果共检测出HCV-1a、1b、2a、3a、3b、6a和6n等7个基因亚型,其中临床患者分别为1.05%(2/191)、66.49%(127/191)、8.90%(17/191)、2.62%(5/191)、2.62%(5/191)、17.80%(34/191)和0.52%(1/191);献血者分别为0.45%(1/222)、41.44%(92/222)、6.76%(15/222)、8.56%(19/222)、4.95%(11/222)、37.39%(83/222)和0.45%(1/222)。2组人群比较,临床患者1b的比例高于献血者(P<0.01),献血者中3a、6a的比例高于临床患者(P<0.05及P<0.01)。结论广东地区临床患者和献血者中的HCV基因亚型主要是HCV-1b和6a,但HCV-1b、3a和6a亚型在2组人群中所占的比例不同。
Objective To understand the distribution of HCV genotypes (subtypes) in clinical patients and blood donors in Guangdong. METHODS: A total of 191 HCV RNA positive samples were collected from clinical patients in Guangdong. 222 HCV RNA positive samples were collected from unpaid blood donors. HCV E1 gene was amplified and sequenced by reverse transcription nested PCR. MEGA5 software was used to construct molecular evolution Tree and genotype it. SPSS16.0 software was used to compare the distribution of different gene subtypes in two groups of people. Results A total of 7 subtypes of HCV-1a, 1b, 2a, 3a, 3b, 6a and 6n were detected in this study. The clinical data were 1.05% (2/191), 66.49% (127/191), 8.90% (1/191), 2.62% (5/191), 17.80% (34/191) and 0.52% (1/191) respectively. The donors were 0.45% (1/222), 41.44 % (92/222), 6.76% (15/222), 8.56% (19/222), 4.95% (11/222), 37.39% (83/222) and 0.45% (1/222). The proportion of 1b in clinical patients was higher than that in blood donors (P <0.01). The proportion of 3a and 6a in blood donors was higher than that in clinical patients (P <0.05 and P <0.01). Conclusion The HCV genotypes in clinical patients and blood donors in Guangdong are mainly HCV-1b and 6a, but the proportions of HCV-1b, 3a and 6a subtypes in the two groups are different.