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以白檀未成熟胚为试材,以改良MS为培养基,研究了白檀未成熟胚的器官发生和植株再生。结果表明:改良MS(mMS)能够很好的诱导愈伤组织,在添加了0.2mg/L 6-BA+0.1mg/L NAA的培养基中,诱导率高达92.5%;胚性愈伤组织分化最佳培养基为mMS+0.25mg/L 6-BA+0.15mg/L NAA,分化率为72.4%;分化出的胚性愈伤组织在空白mMS培养基上继代培养15d,76.6%的组织分化出芽,再转入1/2mMS+1.5%蔗糖培养基上培养20d,芽长至1.5cm。
Using the immature embryos of white sandalwood as test material and the modified MS as medium, the organogenesis and plant regeneration of immature embryos of white sandalwood were studied. The results showed that the improved MS (mMS) could induce callus well, and the induction rate was as high as 92.5% in medium supplemented with 0.2 mg / L 6-BA + 0.1 mg / L NAA. The differentiation of embryogenic callus The best culture medium was mMS + 0.25mg / L 6-BA + 0.15mg / L NAA with a differentiation rate of 72.4%. The differentiated embryogenic callus was subcultured on blank mMS medium for 15 days and 76.6% Differentiated budding, and then transferred to 1 / 2mMS + 1.5% sucrose medium for 20 days, the bud grows to 1.5cm.