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由碎叶——枳橙矮化病毒引起的嫁接口皱折病状,在日本的一些枳壳砧品种或无性系上发现。近年来,由于大力进行高接换种而引起某些病毒包括碎叶——枳橙矮化病毒(TL—CSV)广泛传播。利用40/30℃(白天/夜间温度)处理60天以上可以脱除碎叶病毒,然而温州蜜柑苗木不耐这种热处理温度。由 Murashige 等发现,经 Navarro 等改进的茎尖嫁接技术能够有效地脱除病毒,类病毒及柑桔螺原体(S.Citrl)但碎叶病毒例外。本文报道了利用茎尖嫁接结合短期予热处理脱除温州蜜柑“Niyu”的碎叶病病毒。
Graft folds caused by broken leaf - citrange dwarf virus were found on some Japanese aurantiacus species or clones. In recent years, some viruses including the fragmented leaf-orange blossom dwarf virus (TL-CSV) have been widely spread due to vigorous high-exchange seeding. Debris virus can be removed by treatment at 40/30 ° C (day / night temperature) for more than 60 days, however, Satsuma mandarin seedlings are not resistant to this heat treatment temperature. Murashige et al. Found that the improved shoot tip grafting technique by Navarro et al. Can effectively remove viruses, viroids, and S. citrl but with the exception of the leafy virus. This paper reports the use of shoot tip grafting combined with short-term pre-heat treatment to remove Wenzhou Satsuma Niyu broken leaf virus.