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目的：了解深低温冷冻对部分肝叶的Glisson氏管道及相应肝组织的影响，为临床冷冻处理肝叶管道附近的癌肿提供实验依据。方法：14只小猪，随机分为2组，用冷冻头深低温冷冻实验组动物左肝外叶Glisson管道区域3分钟（冷冻时阻断肝门），对照组仅阻断肝门3分钟。术后观察动物血清AST、ALT、TBILI、ALB的改变，患肝的病理变化，并用彩超了解冷冻肝叶管道的情况。结果：冷冻可造成受冻部位肝实质、胆管及门静脉分支管壁的坏死，而肝动物分支不受影响，术后8周原冷冻区的肝动脉、门静脉分支的管腔仍保持通畅，但患肝叶萎缩、纤维化。动物可耐受上述冷冻。结论：机体可耐受对部分肝叶Glisson氏管道系统二级分支区域的直接深低温冷冻，冷冻可造成受冻部位肝实质的坏死，而达到外科治疗目的。
Objective: To understand the effects of cryogenic freezing on Glisson’s channels and corresponding liver tissues of some lobes of the liver, and to provide experimental evidence for the clinical cryotherapy of cancer adjacent to the lobe of the liver. METHODS: Fourteen piglets were randomly divided into two groups. In the cryopreservation deep cryopreservation experiment group, the Glisson conduit area of the left lateral hepatic artery was blocked for 3 minutes (the hepatic hilum was blocked during freezing). The control group only blocked the portal hepatis for 3 minutes. Postoperatively, the changes of serum AST, ALT, TBILI and ALB were observed, the pathological changes of the liver were observed, and the condition of frozen liver lobe was understood by color ultrasound. Results: Frozen can cause necrosis of hepatic parenchyma, bile duct and portal vein branches in the frozen part, but the branch of hepatic animal is not affected. The lumen of the hepatic artery and portal vein branch of the original frozen zone remained unobstructed for 8 weeks after operation, but it affected Hepatic lobe atrophy and fibrosis. Animals can tolerate the above freezing. Conclusion: The body can tolerate direct cryogenic freezing of the secondary branch of the Glisson’s tube system in some of the hepatic lobes. Freezing can result in necrosis of the hepatic parenchyma in the frozen part, which can achieve surgical treatment.