测定β-谷甾醇和豆甾醇在环己酮中的溶解度,利用β-谷甾醇和豆甾醇在环己酮中的溶解度随温度变化的差异,分别用重结晶法提纯β-谷甾醇和豆甾醇,并对豆甾醇的结晶条件(料液比、结晶温度和养晶时间)进行优化。实验结果表明:经过4次结晶操作,β-谷甾醇的纯度可达到90%;豆甾醇结晶过程中采用料液比(混合植物甾醇与环己酮的比,g/mL)为1∶3.3、结晶温度25℃、养晶5h的条件下,经5次结晶豆甾醇的纯度达到96%。 The solubility of β-sitosterol and stigmasterol in cyclohexanone was determined. By using the difference of solubility of β-sitosterol and stigmasterol in cyclohexanone with temperature, β-sitosterol and stigmasterol were respectively purified by recrystallization , And optimize the crystallization conditions of stigmasterol (solid-to-liquid ratio, crystallization temperature and dwell time). The results showed that the purity of β-sitosterol reached 90% after 4 times of crystallization. The solid-to-liquid ratio (the ratio of phytosterols to cyclohexanone, g / mL) Crystallization temperature 25 ℃, Yang Jing 5h conditions, after 5 times the degree of crystallisation sterol reached 96%.