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目的探讨三水白虎汤对胶原诱导性关节炎模型关节滑膜组织中TNF-α、MMP-1、MMP-3mR-NA表达的影响。方法胶原诱导性关节炎模型鼠33只随机分为3组,分别给予生理盐水、三水白虎汤水煎剂、来氟米特灌胃治疗。灌胃30天后处死大鼠,用原位杂交的方法探测关节局部组织中MMP-1、MMP-3mRNA的表达;用ELISA法检测血清TNF-α含量。结果三水白虎汤组、来氟米特组血清TNF-α的含量显著低于对照组(P<0.05)。SSBH组MMP-1、MMP-3mRNA的表达强度和面积均显著低于对照组(P<0.05)。结论三水白虎汤对胶原诱导性关节炎组织中TNF-α、MMP-1、MMP-3mRNA的表达有一定的抑制作用。
Objective To investigate the effect of Sanshui Baihu Decoction on the expression of TNF-α, MMP-1, MMP-3mR-NA in synovial tissue of collagen-induced arthritis model. Methods Thirty-three rats with collagen-induced arthritis were randomly divided into three groups: saline, Sanshuibaihu decoction, and leflunomide. Rats were killed after intragastric administration for 30 days. The expression of MMP-1 and MMP-3 mRNA in the local tissues of the joints was detected by in situ hybridization. Serum TNF-α levels were measured by ELISA. Results The content of serum TNF-α in Sanshuibaihu decoction group and leflunomide group was significantly lower than that in the control group (P<0.05). The expression and area of MMP-1 and MMP-3 mRNA in SSBH group were significantly lower than those in control group (P<0.05). Conclusion Sanshui Baihu Decoction can inhibit the expression of TNF-α, MMP-1, MMP-3 mRNA in collagen-induced arthritis.