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［目的］研究脐淋巴细胞与ＥＢＶ转化淋巴细胞（ＬＣＩｓ），ＥＢＶ相关细胞核基质蛋白（ＮＭＰｓ）之间的关系，分析鉴定ＥＢＶ感染细胞后的ＮＭＰｓ特异性改变，探讨ＮＭＰｓ与ＥＢＶ转化及肿瘤形成的关系。［方法］ＥＢＶ转化淋巴细胞；改良方法提取ＮＭＰｓ；制备ＮＭＰｓ免疫小鼠血清；ＳＤＳ－ＰＡＧＥ电泳及双波长蛋白薄层扫描方法分析ＮＭＰｓ；免疫蛋白印迹分析ＮＭＰｓ与抗ＮＭＰｓ的阳性血清之间的反应性。［结果］获得ＥＢＶ转化淋巴细胞。获得五种细胞ＮＭＰｓ。各细胞间分子量相同的蛋白约占各自细胞组分的５２．１％～５９．６％。脐淋巴细胞３０ＫＤ～４０ＫＤ蛋白较多，而ＬＣＬｓ和ＥＢＶ相关淋巴细胞中，高分子量的蛋白比较多。－６４ＫＤ ＮＭＰ特异地存在于ＬＣＬｓ及ＥＢＶ相关细胞中。ＮＰＣ病人血清中能够检出抗ＬＣＬｓ的ＮＭＰｓ抗体。［结论］ＬｅｕＬｅｕ－ＯＭｅ和环孢菌素Ａ结合使用，可促进ＥＢＶ转化。低分化的脐淋巴细胞比高分化ＬＣＬｓ有更稳定的核基质网架结构。推测相同分子量ＮＭＰｓ构成核基质的结构成分；ＥＢＶ感染细胞后，ＮＭＰｓ有明显的变化。ＮＭＰｓ的特异性与ＥＢＶ感染高度相关；ＥＢＶ转化与ＮＰＣ的发生有一定关系。
[Objective] To study the relationship between umbilical lymphocytes and EBV transformed lymphocytes (LCIs) and EBV-associated nuclear matrix proteins (NMPs), analyze and identify the specific changes of NMPs after EBV infected cells, and explore the transformation of NMPs and EBV and tumor formation. relationship. [Methods] EBV transformed lymphocytes; Improved methods for extracting NMPs; Preparation of NMPs immunized mice serum; SDS-PAGE electrophoresis and dual-wavelength protein thin layer scanning method for analysis of NMPs; Western blot analysis of the reaction between NMPs and anti-NMPs positive serum Sex. [Results] EBV transformed lymphocytes were obtained. Five cell NMPs were obtained. The proteins with the same molecular weight among the cells accounted for about 52.1% to 59.6% of the respective cell components. Umbilical lymphocytes have more 30KD to 40KD proteins, while LCLs and EBV-associated lymphocytes have more high-molecular-weight proteins. -64KD NMP is specifically present in LCLs and EBV-associated cells. Anti-LCLs of NMPs antibodies can be detected in serum of NPC patients. [Conclusion] The combination of LeuLeu-OMe and cyclosporin A can promote the transformation of EBV. Poorly differentiated umbilical lymphocytes have a more stable nuclear matrix network structure than highly differentiated LCLs. It is speculated that the same molecular weight NMPs constitute the structural components of the nuclear matrix; after EBV infection of cells, NMPs have obvious changes. The specificity of NMPs is highly correlated with EBV infection; EBV transformation has a certain relationship with the occurrence of NPC.