对大鼠压疮缺血-再灌注损伤模型构建及应用的思考

来源 :现代生物医学进展 | 被引量 : 0次 | 上传用户:wbs304
下载到本地 , 更方便阅读
声明 : 本文档内容版权归属内容提供方 , 如果您对本文有版权争议 , 可与客服联系进行内容授权或下架
论文部分内容阅读
目的:为构建大鼠压疮缺血-再灌注损伤模型提供简易模型装置及有效的造模方法。方法:自制简易压疮缺血-再灌注损伤模型装置,对120只雌性大鼠腿部近膝关节骨隆突处进行为期3日的压疮造模,5个循环/日,每个循环分别实现120 min的缺血期及30 min的再灌注期,分别于造模第1 d、2 d、3 d及造模结束第1 d观察大鼠病灶创面的颜色、形态、水肿、结痂、渗出以及大鼠行为学状况、并于造模结束第1 d统计存活率及成模率。结果:造模第1 d,Ⅰ期压疮100只,Ⅱ期压疮17只,死亡3只;造模第2 d,Ⅰ期压疮26只,Ⅱ期压疮84只,死亡7只;造模第3 d,Ⅰ期压疮11只,Ⅱ期压疮95只,死亡4只;造模结束第1 d,Ⅰ期压疮5只,Ⅱ期压疮101只,死亡0只。120只实验大鼠,共14只大鼠死亡,造模存活率达88.33%,Ⅱ期压疮造模成功率达84.17%。结论:本造模装置可有效制备大鼠压疮缺血-再灌注动物模型,具有接近临床,操作简便,无需麻醉,Ⅱ期压疮成模率较高、避免铁片植入带来的皮肤负损伤等优势,这将为皮肤压疮、乃至慢性损伤组织的机制研究、修复及愈合相关研究提供重要理论和实验依据。 OBJECTIVE: To provide a simple model device and an effective modeling method for establishing a model of pressure ulcer ischemia-reperfusion injury in rats. Methods: A simple model of pressure ulcer ischemia-reperfusion injury was established in this study. A pressure ulcer model of 3 days was performed in the legs of 120 female rats. Five cycles / day, The ischemic period of 120 min and the reperfusion period of 30 min were performed. The color, morphology, edema and crusting of the lesion in rats were observed on the 1st, 2nd, 3rd day and 1 day after the model was established respectively. Exudation and behavioral status of rats, and in the first day after the end of modeling the statistical survival rate and the rate of molding. Results: In the first day of model making, 100 stage Ⅰ pressure ulcers and 17 stage Ⅱ pressure ulcers, 3 died; 26 pressure ulcers in stage Ⅱ, 84 pressure ulcers in stage Ⅱ and 7 deaths in stage Ⅱ; In the first 3 days of modeling, there were 11 pressure ulcers in stage Ⅰ, 95 pressure ulcers in stage Ⅱ, and 4 deaths. There were 5 pressure ulcers in stage Ⅰ, stage Ⅰ pressure ulcers and 101 pressure ulcers in stage Ⅱ, and 0 died. 120 experimental rats, a total of 14 rats died, the model survival rate was 88.33%, Ⅱ pressure ulcer modeling success rate of 84.17%. Conclusion: The model device can effectively prepare rat model of pressure ulcer ischemia-reperfusion, which is close to clinical, easy to operate, without anesthesia, high incidence rate of pressure ulcer in stage Ⅱ and avoid the skin caused by iron implantation Negative damage and other advantages, which will provide important theoretical and experimental evidences for the study of mechanism of skin pressure ulcers and chronic injury tissues, repair and healing.
其他文献
广告是广告主通过某种媒介对受众进行某种与商品、劳务或者特定观念有关的信息传递,以期望产生某种效果的活动。原型是人类心理长期的集体无意识的积淀,叙事文本中常使用原型以
目的 观察不同年龄段的扩张型心肌病(DCM)患者应用左西孟旦后的临床疗效。方法 选择2016-01/2017-05在四川省人民医院心衰中心住院治疗诊断为扩张型心肌病患者33例,从年龄、性
以互联网为代表的新媒体的快速发展催生了不少广告创作的新形式。尤其是在微博大行其道的当今,不少适应微博平台传播的广告应运而生,其中以“有奖转发”为代表的新型广告正以
概述了垃圾填埋场中微生物的作用特性, 微生物对有机污染物的降解及其影响因素,介绍了有机污染物降解的两种生化动力学模型: "速度指数模型"和"双曲线速度模型",以及垃圾降解
港珠澳大桥珠海连接线拱北隧道工程预支护体系采用管幕法与人工冻结技术相结合的全新"管幕冻结法"施工.根据工程实际情况,首次提出了在管幕钢管内部布置"圆形主力冻结管"、"
6种五倍子的组织解剖学表现出一致性:表皮层由1层细胞组成,无气孔结构,皮层薄壁组织在五倍子壁结构中占最主要的部分,乳汁道与维管束伴生,贯穿于倍壁,由外至内乳汁道、韧皮部、木质
马克思的现代性思想依次经历了追求"启蒙的现代性"、批判"异化的现代性"和批判"资本的现代性"三个阶段,其发现了与现代性交织共生的资本逻辑,回答了是什么推动了传统向现代的转变、
目的调查某综合医院眼科住院患者的来源、病种分布及变化趋势,为眼病防治和学科建设提供依据。方法对2000年-2009年7997例眼科住院患者按疾病分类等指标进行统计。结果患者来
以小扁豆为原料,利用浸泡和萌芽富集γ-氨基丁酸。通过单因素和正交试验研究浸泡时间和温度、萌芽时间和温度对小扁豆中γ-氨基丁酸合成的影响,从而确定最佳工艺条件。结果表明