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目的:为构建大鼠压疮缺血-再灌注损伤模型提供简易模型装置及有效的造模方法。方法:自制简易压疮缺血-再灌注损伤模型装置,对120只雌性大鼠腿部近膝关节骨隆突处进行为期3日的压疮造模,5个循环/日,每个循环分别实现120 min的缺血期及30 min的再灌注期,分别于造模第1 d、2 d、3 d及造模结束第1 d观察大鼠病灶创面的颜色、形态、水肿、结痂、渗出以及大鼠行为学状况、并于造模结束第1 d统计存活率及成模率。结果:造模第1 d,Ⅰ期压疮100只,Ⅱ期压疮17只,死亡3只;造模第2 d,Ⅰ期压疮26只,Ⅱ期压疮84只,死亡7只;造模第3 d,Ⅰ期压疮11只,Ⅱ期压疮95只,死亡4只;造模结束第1 d,Ⅰ期压疮5只,Ⅱ期压疮101只,死亡0只。120只实验大鼠,共14只大鼠死亡,造模存活率达88.33%,Ⅱ期压疮造模成功率达84.17%。结论:本造模装置可有效制备大鼠压疮缺血-再灌注动物模型,具有接近临床,操作简便,无需麻醉,Ⅱ期压疮成模率较高、避免铁片植入带来的皮肤负损伤等优势,这将为皮肤压疮、乃至慢性损伤组织的机制研究、修复及愈合相关研究提供重要理论和实验依据。
OBJECTIVE: To provide a simple model device and an effective modeling method for establishing a model of pressure ulcer ischemia-reperfusion injury in rats. Methods: A simple model of pressure ulcer ischemia-reperfusion injury was established in this study. A pressure ulcer model of 3 days was performed in the legs of 120 female rats. Five cycles / day, The ischemic period of 120 min and the reperfusion period of 30 min were performed. The color, morphology, edema and crusting of the lesion in rats were observed on the 1st, 2nd, 3rd day and 1 day after the model was established respectively. Exudation and behavioral status of rats, and in the first day after the end of modeling the statistical survival rate and the rate of molding. Results: In the first day of model making, 100 stage Ⅰ pressure ulcers and 17 stage Ⅱ pressure ulcers, 3 died; 26 pressure ulcers in stage Ⅱ, 84 pressure ulcers in stage Ⅱ and 7 deaths in stage Ⅱ; In the first 3 days of modeling, there were 11 pressure ulcers in stage Ⅰ, 95 pressure ulcers in stage Ⅱ, and 4 deaths. There were 5 pressure ulcers in stage Ⅰ, stage Ⅰ pressure ulcers and 101 pressure ulcers in stage Ⅱ, and 0 died. 120 experimental rats, a total of 14 rats died, the model survival rate was 88.33%, Ⅱ pressure ulcer modeling success rate of 84.17%. Conclusion: The model device can effectively prepare rat model of pressure ulcer ischemia-reperfusion, which is close to clinical, easy to operate, without anesthesia, high incidence rate of pressure ulcer in stage Ⅱ and avoid the skin caused by iron implantation Negative damage and other advantages, which will provide important theoretical and experimental evidences for the study of mechanism of skin pressure ulcers and chronic injury tissues, repair and healing.