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[目的]观察MTHFR mRNA在砷中毒患者、病区对照和非病区对照人群中的表达及其与尿砷甲基化水平的关系。[方法]选取饮水型砷中毒中重度患者、轻度患者、病区对照和非病区对照各6例,采用实时定量RT-PCR技术测定研究对象的淋巴细胞中MTHFR mRNA表达,采用AFS-9130、SAP-10检测尿iAs、MMA、DMA、TAs含量。按PMI=(MMA+DMA)/TAs,SMI=DMA/(MMA+DMA)分别计算一甲基化指数和二甲基化指数。[结果]中重度组,轻度组、病区对照组人群iAs,MMA,DMA,TAs,PMI,MTHFR mRNA均显著高于非病区对照人群(P﹤0.05);MTH-FR mRNA分别与MMA%(r=0.511,P=0.010)和PMI(r=0.419,P=0.041)呈显著正相关。[结论]慢性砷中毒可能与砷暴露引起MTHFR mRNA表达增高进而引起甲基供体增多,使PMI水平增高导致生成MMA增多有关。
[Objective] To observe the expression of MTHFR mRNA in arsenic poisoning patients, ward-controlled and non-ward-control cohorts and its relationship with urinary arsenic methylation. [Methods] Sixty-four patients with moderate to severe acute drinking water, mild patients, ward-controlled and non-ward controls were selected. Real-time quantitative RT-PCR was used to detect the expression of MTHFR mRNA in lymphocytes. AFS-9130 , SAP-10 urine iAs, MMA, DMA, TAs content. The methylation index and the dimerization index were calculated as PMI = (MMA + DMA) / TAs, SMI = DMA / (MMA + DMA), respectively. [Results] The levels of iAs, MMA, DMA, TAs, PMI and MTHFR in moderate-severe group, mild group and ward control group were significantly higher than those in non-ward control group (P <0.05) % (r = 0.511, P = 0.010) and PMI (r = 0.419, P = 0.041). [Conclusion] Chronic arsenic poisoning may be related to the increase of MTHFR mRNA expression caused by arsenic exposure, which in turn leads to the increase of methyl donor and the increase of PMI, leading to the increase of MMA.