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研究主要分析氮元素对宁夏平罗盐渍化枸杞园土壤中氨氧化微生物(氨氧化细菌和氨氧化古菌)的影响。实验共设八个处理:(1)C(不施氮肥,不施脱硫废弃物,原始荒地);(2)不施氮肥(N0);(3)施氮肥25 kg hm~(-2)(N25);(4)施氮肥50 kg hm~(-2)(N50);(5)施氮肥100kg hm~(-2)(N100);(6)施氮肥200 kg hm~(-2)(N200);(7)施氮肥400 kg hm~(-2)(N400);(8)施氮肥800 kg hm~(-2)(N800),在N0-N800处理施用脱硫废弃物3.72×104kg hm~(-2)。2011年8月采集0~20 cm土样。结果显示:脱硫废弃物和氮肥配合施加对土壤理化性质产生了显著影响;NO_3~--N和NH_4~+-N含量在施氮处理中相对于原始样地和不施氮处理组都有显著的升高,微生物生物量和细菌和氨氧化细菌多样性指数在施氮400 kg hm~(-2)达到最大值,施氮肥400 kg hm~(-2)是促进微生物量和群落多样性增加的最佳施用量。实时荧光定量PCR结果显示:氨氧化细菌(AOB)丰度在施氮肥400 kg hm~(-2)和800 kg hm~(-2)显著高于其它处理,脱硫废弃物和氮肥配合施用对AOB的丰度具有叠加效应。相关性分析表明:NH_4~+-N与总磷脂脂肪酸(PLFA)、细菌PLFA、革兰氏阳性菌(G+)、革兰氏阴性菌(G-)、真菌/细菌(F/B)、微生物碳(MBC)、微生物氮(MBN)及16S r RNA基因拷贝数、AOB的基因拷贝数都显著相关。因此,铵态氮是该地区微生物群落可利用的有效氮素。
The study mainly analyzed the effects of nitrogen on the ammonia oxidizing bacteria (ammonia oxidizing bacteria and ammonia oxidizing archaea) in the soil of Lycium Barbarum salinized in Ningxia. A total of eight experiments were conducted: (1) C (no nitrogen fertilizer, no desulfurization waste and virgin land); (2) no nitrogen fertilizer (N0); (3) 25 kg hm -2 N25); (4) 50 kg hm -2 (N50) of nitrogen fertilizer; (5) 100 kg hm -2 (N100) of nitrogen fertilizer; (6) 200 kg hm -2 N200); (7) 400 kg hm -2 (N400) of nitrogen fertilizer; (8) 800 kg hm -2 (N800) of nitrogen fertilizer; and 3.72 × 104 kg hm ~ (-2). August 2011 collected 0 ~ 20 cm soil samples. The results showed that the combination of desulfurized waste and nitrogen fertilizers had a significant effect on the physical and chemical properties of soil. The content of NO 3 - -N and NH 4 + -N in the treatment of nitrogen application was significantly higher than that of the original sample and no nitrogen application , Microbial biomass and bacterial and ammoniacal bacterial diversity index reached the maximum at nitrogen application rate of 400 kg hm -2, and nitrogen application rate of 400 kg hm -2 increased microbial biomass and community diversity The best application rate. The results of real-time quantitative PCR showed that the abundance of AOB was significantly higher than that of other treatments when the nitrogen application rates were 400 kg hm -2 and 800 kg hm -2, and the AOB The abundance has a superposition effect. Correlation analysis showed that NH_4 ~ + -N interacted with PLFA, bacterial PLFA, Gram-positive bacteria, G-, F / B, Carbon MBC, MBN, 16S rRNA gene copy number and AOB gene copy number were all significantly correlated. Therefore, ammonium nitrogen is an available nitrogen available to the microbial communities in the area.