在附加６ＢＡ３０ｍｇ／Ｌ、ＩＢＡ００５ｍｇ／Ｌ的ＭＳ再生培养基中加入１～１００μｍｏｌ／ＬＡｇＮＯ３，均显著促进苹果叶片再生不定芽，但以１μｍｏｌ／Ｌ浓度效果最好。与对照相比，在培养早期（２～５ｄ），１μｍｏｌ／ＬＡｇＮＯ３对乙烯的抑制率为１６％～２０％，在培养中后期则为２５％～４０％，因而显著降低了各培养时期，特别是中后期（芽分化期）培养容器中乙烯的浓度，降低了乙烯对芽发生的抑制，从而促进不定芽的发生 Addition of 1 ~ 100μmol / LAgNO3 to MS regeneration medium supplemented with 6BA30mg / L and IBA005mg / L significantly promoted the regeneration of adventitious buds in apple leaves, but the effect of 1μmol / L was the best. Compared with the control, 1μmol / LAgNO3 inhibited ethylene at 16% -20% in the early stage of culture (2 ~ 5 days) and 25% ~ 40% in the late stage of culture, which significantly reduced the incubation period, especially Is the concentration of ethylene in the culture vessel in the late stage (bud differentiation stage), which reduces the inhibition of ethylene on the occurrence of buds and thus promotes the occurrence of adventitious buds