目的:探讨细菌性肺炎患儿血清白细胞介素32(IL-32)水平及其与疾病严重程度的关系。方法:选择台州市肿瘤医院2019年1-12月收治的细菌性肺炎患儿300例为肺炎组、健康体检儿童300例为对照组。采用双抗体夹心酶联免疫吸附试验测定血清IL-32、IL-6、IL-8、肿瘤坏死因子α(TNF-α)水平。结果:肺炎组血清IL-32[(212.46±16.74)ng/L]和IL-6[(0.29±0.06)μg/L]、IL-8[(0.41±0.08)μg/L]、TNF-α[(1.15±0.11)μg/L]水平高于对照组[(116.51±14.52)ng/L、(0.07±0.02)μg/L、(0.09±0.03)μg/L、(0.14±0.03)μg/L](均n P<0.05)。肺炎患儿恢复期血清IL-32[(128.76±15.42)ng/L]和IL-6[(0.15±0.04)μg/L]、IL-8[(0.16±0.05)μg/L]、TNF-α[(0.29±0.06)μg/L]水平低于早期[(212.46±16.74)ng/L、(0.29±0.06)μg/L、(0.41±0.08)μg/L、(1.15±0.11)μg/L](均n P<0.05)。重症组血清IL-32[(232.16±16.02)ng/L]和IL-6[(0.37±0.08)μg/L]、IL-8[(0.54±0.09)μg/L]、TNF-α[(1.42±0.15)μg/L]水平高于非重症组[(184.26±16.31)ng/L、(0.23±0.05)μg/L、(0.26±0.06)μg/L、(0.79±0.12)μg/L](均n P<0.05)。肺炎患儿血清IL-32水平与IL-6(n r＝0.602)、IL-8(n r＝0.624)、TNF-α(n r＝0.593)水平呈正相关(均n P<0.05)。n 结论:细菌性肺炎患儿血清IL-32水平升高可反映细菌性肺炎患儿的治疗效果、严重程度和炎性反应程度。“,”Objective:To investigate the serum interleukin-32(IL-32) level in children with bacterial pneumonia and its relationship with the severity of the disease.Methods:From January 2019 to December 2019, 300 children with bacterial pneumonia in Taizhou Cancer Hospital were selected as the pneumonia group, and 300 healthy children in our hospital were selected as control group.The serum levels of IL-32, IL-6, IL-8 and tumor necrosis factor-α(TNF-α) were measured by double antibody sandwich enzyme-linked immunosorbent assay.Results:The serum levels of IL-32[(212.46±16.74)ng/L], IL-6[(0.29±0.06)μg/L], IL-8[(0.41±0.08)μg/L] and TNF-α[(1.15±0.11)μg/L] in the pneumonia group were higher than those in the control group[(116.51±14.52)ng/L, (0.07±0.02)μg/L, (0.09±0.03)μg/L, (0.14±0.03)μg/L](alln P<0.05). The serum levels of IL-32[(128.76±15.42)ng/L], IL-6[(0.15±0.04)μg/L], IL-8[(0.16±0.05)μg/L] and TNF-α[(0.29±0.06)μg/L] in children with pneumonia during recovery were lower than those in early stage[(212.46±16.74)ng/L, (0.29±0.06)μg/L, (0.41±0.08)μg/L, (1.15±0.11)μg/L](alln P<0.05). The levels of serum IL-32[(232.16±16.02)ng/L], IL-6[(0.37±0.08)μg/L], IL-8[(0.54±0.09)μg/L], TNF-α[(1.42±0.15)μg/L] in the severe pneumonia group were higher than those in the non-severe pneumonia group[(184.26±16.31)ng/L, (0.23±0.05)μg/L, (0.26±0.06)μg/L, (0.79±0.12)μg/L](alln P<0.05). There were positive correlation between serum IL-32 level and IL-6(n r=0.602), IL-8(n r=0.624), TNF-α(n r=0.593) levels in children with pneumonia(all n P<0.05).n Conclusion:The serum IL-32 level is elevated in children with bacterial pneumonia.The serum IL-32 level can reflect the therapeutic effect, severity and inflammatory response of children with bacterial pneumonia.