目的：探讨抗独特型疫苗主动免疫治疗鼻咽癌病人的抗肿瘤效应。方法：用两株具有鼻咽癌相关抗原内影像的抗独特型单克隆抗体２ Ｈ４ 、５ Ｄ３ ，经氢氧化铝凝胶沉淀法制备成抗独特型疫苗 Ａｌｕｍ２ Ｈ４ 、 Ａｌｕｍ５ Ｄ３ ，对１９ 例晚期鼻咽癌放疗病人作主动免疫治疗，９ 例放疗加生理盐水注射为对照组。用 Ｅ Ｌ Ｉ Ｓ Ａ 检测治疗前后病人血清抗体和细胞因子水平。用原位 Ｎｏｒｔｈｅｒｎ 杂交检测外周血单个核细胞（ Ｐ Ｂ Ｍ Ｃ） Ｉ Ｌ２ ｍ Ｒ Ｎ Ａ 的表达。结果： 接受 Ａｌｕｍ２ Ｈ４ 或 Ａｌｕｍ５ Ｄ３ 治疗的病人无一例有过敏或其他毒副反应， 血清中抗抗独特型抗体（ Ａｂ３） 、抗肿瘤抗体（ Ａｂ１’） 水平均有不同程度的增高，但也产生了人抗鼠抗体（ Ｈ Ａ Ｍ Ａ） 。血清细胞因子 Ｔ Ｎ Ｆα、 Ｉ Ｆ Ｎγ和 Ｉ Ｌ２ 水平在大多数治疗组病人中升高。而对照组 Ａｂ１＇ 、 Ｉ Ｆ Ｎγ、 Ｔ Ｎ Ｆα及 Ｉ Ｌ２ 血清水平均未升高。病人血清的 Ｉ Ｌ２ 含量与 Ｐ Ｂ Ｍ Ｃ Ｉ Ｌ２ ｍ Ｒ Ｎ Ａ 的表达呈正相关关系（ｒ＝ ０．８８２９） 。结论：（１） 疫苗化的２ Ｈ４ 和５ Ｄ３ 用于晚期鼻咽癌病人的主动免疫治疗是安全的。（２） 抗独特型疫苗 Objective: To investigate the anti-tumor effect of anti-idiotype vaccine active immunotherapy for nasopharyngeal carcinoma patients. METHODS: Anti-idiotypic vaccines Alum2 H4 and Alum5 D3 were prepared from two strains of anti-idiotypic monoclonal antibodies 2 H4 and 5 D3 with nasopharyngeal carcinoma-associated antigens and precipitated by aluminum hydroxide gel. Nineteen patients with advanced nasopharyngeal carcinoma received active immunotherapy, and nine patients received radiotherapy plus normal saline injection as a control group. The serum antibody and cytokine levels were measured before and after treatment with E L I S A . The expression of I L  2 m R N A in peripheral blood mononuclear cells (P B M C) was detected by in situ Northern blotting. Results: None of the patients treated with Alum2 H4 or Alum5 D3 had any allergic or other side effects. Anti-anti-idiotypic antibodies (Ab3) and anti-tumor antibodies (Ab1′) were increased in serum to varying degrees. However, human anti-mouse antibodies (H A M A) have also been produced. Serum cytokines T N F  α, I F N  γ, and I L  2 levels were elevated in most treatment groups. However, the serum levels of Ab1’, I F N γ, T N F  α and I L  2 in the control group did not increase. The serum level of I L  2 was positively correlated with the expression of P B M C I L  2 m R N A (r = 0.8829). Conclusions: (1) Active immunization of vaccines for 2H4 and 5D3 in patients with advanced nasopharyngeal carcinoma is safe. (2) Anti-idiotype vaccine